Thermophilin 13, a nontypical antilisterial poration complex bacteriocin, that functions without a receptor.

The Journal of Biological Chemistry
O MarcisetB Poolman

Abstract

A novel broad host range antimicrobial substance, Thermophilin 13, has been isolated and purified from the growth medium of Streptococcus thermophilus. Thermophilin 13 is composed of the antibacterial peptide ThmA (Mr of 5776) and the enhancing factor ThmB (Mr of 3910); the latter peptide increased the activity of ThmA approximately 40 x. Both peptides are encoded by a single operon, and an equimolar ratio was optimal for Thermophilin 13 activity. Despite the antilisterial activity of Thermophilin 13, neither ThmA nor ThmB contain the YGNGV-C consensus sequence of Listeria-active peptides, and post-translational modifications comparable to that in the lantibiotics are also absent. Mass spectrometry did reveal the apparent oxidation of methionines in ThmA, which resulted in a peptide that could not be enhanced any longer by ThmB, whereas the intrinsic bactericidal activity was normal. Thermophilin 13 dissipated the membrane potential and the pH gradient in liposomes, and this activity was independent of membrane components from a sensitive strain (e.g. lipid or proteinaceous receptor). Models of possible poration complexes formed are proposed on the basis of sequence comparisons, structure predictions, and the functional analysi...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Feb 1, 1991·Applied and Environmental Microbiology·M J van BelkumG Venema
May 7, 1990·Biochimica Et Biophysica Acta·C E Dempsey
Jul 21, 1988·Biochimica Et Biophysica Acta·I R MellorM S Sansom
Jan 1, 1989·Critical Reviews in Microbiology·W N KoningsA J Driessen
Nov 1, 1988·Rapid Communications in Mass Spectrometry : RCM·T R CoveyJ Henion
Jun 15, 1995·European Journal of Biochemistry·H G SahlG Bierbaum
Jun 1, 1995·Microbiological Reviews·R W JackB Ray
Jan 1, 1993·Methods in Enzymology·A J Driessen, W N Konings
Sep 1, 1993·FEMS Microbiology Reviews·T R Klaenhammer
Feb 20, 1993·Journal of Molecular Biology·N Unwin
Feb 1, 1996·Journal of Bacteriology·G MollA J Driessen
Nov 1, 1995·Protein Science : a Publication of the Protein Society·C N PaceT Gray
Sep 1, 1993·Applied and Environmental Microbiology·M E Bruno, T J Montville
Aug 1, 1996·Applied and Environmental Microbiology·B GonzalezW N Konings

❮ Previous
Next ❯

Citations

Aug 10, 2000·Biopolymers·I F Nes, H Holo
Jul 29, 2005·Current Microbiology·Stefanie E Gilbreth, George A Somkuti
Jul 17, 2007·Current Microbiology·George A Somkuti, Stefanie E Gilbreth
Nov 28, 2012·Biotechnology Letters·John A Renye, George A Somkuti
May 12, 2001·FEMS Microbiology Reviews·O McAuliffeC Hill
Nov 9, 2002·Biochimie·Sylvie GarneauJohn C Vederas
Dec 22, 1999·Current Opinion in Chemical Biology·D G McCaffertyR Kruger
Oct 6, 2005·Nature Reviews. Microbiology·Paul D CotterR Paul Ross
Sep 6, 2000·Journal of Applied Microbiology·M C Martínez-CuestaT Requena
Jul 10, 2001·Journal of Applied Microbiology·A VaughanD van Sinderen
Jul 18, 2006·Nucleic Acids Research·Anne de JongOscar P Kuipers
Jun 29, 2010·Applied and Environmental Microbiology·Eileen F O'SheaColin Hill
Mar 6, 2007·Applied and Environmental Microbiology·Camilla OppegårdJon Nissen-Meyer
Dec 9, 2003·Applied and Environmental Microbiology·Anne VaughanDouwe Van Sinderen
Aug 19, 2007·Journal of Bacteriology·Laetitia FontainePascal Hols
Nov 14, 2006·Journal of Bacteriology·Ingolf F NesHelge Holo
Feb 16, 2010·Journal of Bacteriology·Morten KjosDzung B Diep
Sep 7, 2001·Journal of Bacteriology·J HoskinsJ I Glass
Sep 11, 2007·Journal of Molecular Microbiology and Biotechnology·Camilla OppegårdJon Nissen-Meyer
Jul 28, 2012·Prikladnaia biokhimiia i mikrobiologiia·L G StoianovaA I Netrusov
Jun 24, 2008·Critical Reviews in Biotechnology·Antonio GalvezNabil Ben Omar
Feb 11, 2016·Applied Microbiology and Biotechnology·Patricia Alvarez-SieiroOscar P Kuipers
Dec 1, 2015·Biochimica Et Biophysica Acta·Xu ZhangPinglan Li
Jun 17, 2008·Biochimica Et Biophysica Acta·Nina FimlandPer Eugen Kristiansen
Jan 12, 2008·Biochimica Et Biophysica Acta·Per RognePer Eugen Kristiansen
Feb 5, 2009·Journal of Applied Microbiology·T KabukiH Nakajima
Nov 26, 2009·Journal of Applied Microbiology·J A Renye, G A Somkuti
Apr 20, 2016·Frontiers in Microbiology·Kevin EganPaul D Cotter
Sep 10, 2013·Food Microbiology·S MessaoudiX Dousset
Aug 16, 2016·Biotechnology Letters·J A RenyeD H Steinberg
Apr 13, 2010·Probiotics and Antimicrobial Proteins·Jon Nissen-MeyerPer Eugen Kristiansen
Aug 8, 2018·FEMS Microbiology Reviews·Jeella Z AcedoMarco J van Belkum

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.