Thermophoretic Detection of Exosomal microRNAs by Nanoflares.

Journal of the American Chemical Society
Junxiang ZhaoJiashu Sun

Abstract

Exosomal microRNAs (miRNAs) are reliable and noninvasive biomarkers for the early diagnosis of cancer. Yet, accurate and feasible detection of exosomal miRNAs is often hampered by the low abundance of miRNAs in exosomes and the requirement for RNA extraction in large sample volumes. Here we show a thermophoretic sensor implemented with nanoflares for in situ detection of exosomal miRNAs, without resorting to either RNA extraction or target amplification. Thermophoretic accumulation of nanoflare-treated exosomes leads to an amplified fluorescence signal upon the binding of exosomal miRNAs to nanoflares, allowing for direct and quantitative measurement of exosomal miRNAs down to 0.36 fM in 0.5 μL serum samples. One of the best markers, exosomal miR-375, showed an accuracy of 85% for detection of estrogen receptor-positive breast cancer at early stages (stages I, II). This work provides a feasible tool to improve the diagnosis of cancer.

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Citations

Nov 13, 2020·International Journal of Cancer. Journal International Du Cancer·Shiyu LiKongming Wu
Jan 21, 2021·International Journal of Nanomedicine·Kai ChenXin Wang
Jan 8, 2021·Journal of Experimental & Clinical Cancer Research : CR·Xiangdong XuYiquan Ke
Feb 27, 2021·Analytical Chemistry·He YanYong Zeng
Mar 6, 2021·Chemical Reviews·Lingling WuWeihong Tan
Mar 19, 2021·ACS Nano·Shaofeng LiuHong-Bo Sun
May 21, 2021·Journal of Extracellular Vesicles·Yaxuan LiangMengrou Lu
Jun 24, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bicong ZhouChangmin Yu
Jun 24, 2020·Journal of the American Chemical Society·Sasha B EbrahimiChad A Mirkin
Sep 1, 2021·Advanced Science·Li MinShutao Wang

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