Thermoreversible gelation polymer induces the emergence of hepatic stem cells in the partially injured rat liver

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Masaki NagayaToshihiro Mitaka

Abstract

Focal injury of the adult liver causes formation of granulomatous tissue and fibrosis. When thermoreversible gelation polymer (TGP) was applied to such defects of the rat liver, complete recovery of hepatic tissues was observed without granulation. We analyzed the mechanism of the regeneration. TGP is a chemically synthesized biocompatible polymer material whose sol-gel transition is reversible by changing the temperature. Cooled TGP was poured into a penetration lesion of the rat liver. Immunohistochemistry and polymerase chain reaction were carried out using tissues and cultured cells isolated from ductular structures. Immunocytochemical and ultrastructural analyses were also conducted. Seven days after TGP treatment, ductular reactions were observed around the wound and ductules elongated to the injured area. Cells in the structures were alpha-fetoprotein (AFP) positive, albumin+, CK19+, c-Kit+, and Thyl+. Hepatocyte-like cells possessing glycogen appeared around the tips of the ductules from day 9. The defect was completely replaced with hepatocytes by day 28. Cells isolated from the ductules expressed Musashi-1, c-Kit, Thyl, AFP, albumin, transferrin, connexin 43, and CK19. When the cultured cells were covered by TGP, they...Continue Reading

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Citations

Sep 18, 2007·Tissue Engineering·Kirstin LinkeHeike Mertsching
Sep 14, 2017·Cell Transplantation·Noboru SuzukiTomoko Suzuki
Nov 24, 2018·International Journal of Urology : Official Journal of the Japanese Urological Association·Surya P VaddiSamuel Jk Abraham

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