Thermosiphon-based PCR reactor: experiment and modeling

Analytical Chemistry
Zongyuan ChenHaim H Bau

Abstract

A self-actuated, flow-cycling polymerase chain reaction (PCR) reactor that takes advantage of buoyancy forces to continuously circulate reagents in a closed loop through various thermal zones has been constructed, tested, and modeled. The heating required for the PCR is advantageously used to induce fluid motion without the need for a pump. Flow velocities on the order of millimeters per second are readily attainable. In our preliminary prototype, we measured a cross-sectionally averaged velocity of 2.5 mm/s and a cycle time of 104 s. The flow velocity is nearly independent of the loop's length, making the device readily scalable. Successful amplifications of 700- and 305-bp fragments of Bacillus cereus genomic DNA have been demonstrated. Since the device does not require any moving parts, it is particularly suitable for miniature systems.

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Citations

Apr 23, 2008·Analytical and Bioanalytical Chemistry·Liza LamHiroyuki Noji
Jan 13, 2006·Biomedical Microdevices·Jing WangHaim H Bau
Nov 1, 2008·Biomedical Microdevices·Liang-Ju ChienGwo-Bin Lee
Mar 16, 2011·Journal of Visualized Experiments : JoVE·Radha MudduVictor M Ugaz
Apr 10, 2007·Clinics in Laboratory Medicine·Nitin Agrawal, Victor M Ugaz
Feb 8, 2011·Biotechnology Journal·Qingqing CaoCatherine Klapperich
Feb 1, 2006·Journal of Microelectromechanical Systems : a Joint IEEE and ASME Publication on Microstructures, Microactuators, Microsensors, and Microsystems·Shifeng LiShaochen Chen
Dec 6, 2005·Biotechnology Advances·Chunsun ZhangWenling Zheng
Aug 25, 2009·Biosensors & Bioelectronics·Heiko DuwenseeGerd-Uwe Flechsig
Aug 17, 2016·Biosensors·Brigitte BruijnsHan Gardeniers
Jul 7, 2005·Advances in Dental Research·D MalamudP Corstjens
Jun 9, 2016·SLAS Technology·Xianbo QiuShizhi Qian
May 19, 2006·Expert Review of Molecular Diagnostics·Luc Bissonnette, Michel G Bergeron
May 1, 2007·Angewandte Chemie·Nitin AgrawalVictor M Ugaz
Oct 20, 2005·Lab on a Chip·Zongyuan ChenHaim H Bau
Mar 31, 2006·Lab on a Chip·Javier Atencia, David J Beebe
Dec 21, 2006·Lab on a Chip·Curtis D ChinSamuel K Sia
Jul 7, 2015·PloS One·Grace WongSeason Wong
Jul 2, 2020·Critical Reviews in Clinical Laboratory Sciences·Vinoth Kumar RajendranAnwar Sunna
Oct 10, 2012·Lab on a Chip·Aashish PriyeVictor M Ugaz
Feb 9, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Lin Gui, Carolyn L Ren

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