PMID: 9631543Jun 19, 1998Paper

Thermostable alpha-galactosidase from Thermotoga neapolitana: cloning, sequencing and expression

FEMS Microbiology Letters
M R KingB M Chassy

Abstract

A gene encoding a thermostable alpha-galactosidase from the hyperthermophile Thermotoga neapolitana was cloned and sequenced. Sequence analysis showed that the 552-amino acid protein is similar to Escherichia coli Raf type alpha-galactosidase and belongs to Family 36 of the glycosyl hydrolases. Recombinant alpha-galactosidase expressed in E. coli has a molecular mass of ca. 61 kDa, and an optimum activity at 93 degrees C at pH 7.0. The enzyme is highly thermostable and retains 75% of activity after heating to 80 degrees C for 4 h. The potential application of the enzyme to high temperature processing of soy molasses has been demonstrated.

References

Apr 1, 1991·Journal of General Microbiology·J Aduse-OpokuR R Russell
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jun 1, 1996·The Biochemical Journal·B Henrissat, A Bairoch

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Citations

Apr 2, 2008·Annals of the New York Academy of Sciences·Amy L VanfossenRobert M Kelly
Sep 1, 2006·Food Microbiology·J Leonel Ochoa-Solano, Jorge Olmos-Soto
Oct 27, 2001·Applied and Environmental Microbiology·J Coombs, J E Brenchley
Aug 14, 2013·Critical Reviews in Biotechnology·Priti KatroliaZhengqiang Jiang

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