Thermostable DNA immobilization and temperature effects on surface hybridization.

Langmuir : the ACS Journal of Surfaces and Colloids
Dongbiao GeRastislav Levicky

Abstract

Monolayer films of nucleic acids on solid supports are encountered in a range of diagnostic and bioanalytical applications. These applications often rely on elevated temperatures to improve performance; moreover, studies at elevated temperatures can provide fundamental information on layer organization and functionality. To support such applications, this study compares thermostability of oligonucleotide monolayers immobilized to gold by first coating the gold with a nanometer-thick film (an "anchor layer") of a polymercaptosiloxane, to which DNA oligonucleotides are subsequently tethered through maleimide-thiol conjugation, with thermostability of monolayers formed via widely used attachment through a terminal thiol moiety on the DNA. The temperature range covered is from 25 to 90 °C. After confirming stability of immobilization and, more importantly, retention of hybridization activity even under the harshest conditions investigated, these thermostable films are used to demonstrate measurements of (1) reversible surface melting transitions and (2) temperature dependence of competitive hybridization, when fully matched and mismatched sequences compete for binding to immobilized DNA oligonucleotides. The competitive hybridizati...Continue Reading

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Citations

Oct 11, 2012·Journal of the American Chemical Society·Irina Belozerova, Rastislav Levicky
Oct 16, 2015·Chemical Communications : Chem Comm·Wanqiong QiaoRastislav Levicky
Apr 25, 2015·Physical Chemistry Chemical Physics : PCCP·Zhiguo LiJulianne M Gibbs-Davis
Jun 20, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Jaroslav FilipPeter Kasak
Mar 1, 2018·Annual Review of Analytical Chemistry·Dekai YeChunhai Fan
Jan 14, 2021·Nanomaterials·Marie-Charlotte HornyJean-Michel Siaugue
Mar 13, 2021·The Journal of Physical Chemistry. B·Eshan Treasurer, Rastislav Levicky
Feb 18, 2017·Analytical Chemistry·Lucyano J A MacedoAric Opdahl
Dec 10, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Hao-Chun Chiang, Rastislav Levicky
Jul 17, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Han Ju LeeT Randall Lee
Dec 3, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Tyler J PettyAric Opdahl

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