PMID: 2492226Jan 2, 1989Paper

Thermostable DNA polymerase from the archaebacterium Sulfolobus acidocaldarius. Purification, characterization and immunological properties

European Journal of Biochemistry
C ElieP Forterre

Abstract

We have purified to near homogeneity a DNA polymerase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. Sodium dodecyl sulfate gel electrophoresis of the purified enzyme revealed a polypeptide of 100 kDa. On the basis of a Stokes radius of 4.2 nm and a sedimentation coefficient of 6 S, the purified enzyme has an estimated molecular mass of 109 kDa. These results are consistent with the enzyme being a monomer of 100 kDa. In addition a polyclonal antiserum, obtained by injection of the electroeluted 100-kDa polypeptide into a rabbit, specifically neutralized the DNA-polymerase activity. The enzyme is sensitive to both N-ethylmaleimide and 2',3'-dideoxyribosylthymine triphosphate and resistant to aphidicolin. The purified DNA polymerase has neither exonuclease nor primase activities. In our in vitro conditions, the enzyme is thermostable up to 80 degrees C and is active between 55 degrees C and 85 degrees C in the presence of activated calf-thymus DNA.

References

May 15, 1987·European Journal of Biochemistry·H P ZabelJ Winter
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S B Zimmerman, B Harrison
Oct 15, 1985·European Journal of Biochemistry·M Nakayama, M Kohiyama
Aug 1, 1984·Journal of Bacteriology·P ForterreM Kohiyama
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J Shlomai, A Kornberg

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Citations

Jan 1, 1995·World Journal of Microbiology & Biotechnology·M CiaramellaM Rossi
Jan 1, 1995·World Journal of Microbiology & Biotechnology·C Leuschner, G Antranikian
Feb 11, 1993·Nucleic Acids Research·M Duguet
Jan 1, 1990·Annals of the New York Academy of Sciences·H D SimpsonR M Daniel
May 4, 2017·Nature Communications·Jiangyu YanStephen D Bell
Aug 10, 1999·Journal of Molecular Biology·M De FeliceF M Pisani
Jul 1, 1992·Journal of Bacteriology·E SellmannP Westermann
Apr 1, 1997·Journal of Bacteriology·D R EdgellW F Doolittle

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