Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation

Proteomics
Marek SebelaAndrej Shevchenko

Abstract

Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins.

References

Dec 1, 1987·Analytical Biochemistry·E F RobertsonH C Reeves
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Dec 1, 1981·Journal of Inorganic Biochemistry·T Vajda, A Garai
Aug 17, 1999·Current Opinion in Biotechnology·G DeSantis, J B Jones
May 18, 2000·Analytical Chemistry·A ShevchenkoK G Standing
Mar 28, 2003·Analytical Chemistry·Jan HavlisAndrej Shevchenko
Jul 3, 2003·Journal of the American Society for Mass Spectrometry·Ruedi Aebersold
Mar 23, 2004·Molecular & Cellular Proteomics : MCP·Jesper V OlsenMatthias Mann
May 29, 2004·Analytical Chemistry·Jan Havlis, Andrej Shevchenko
Sep 7, 2004·Electrophoresis·Mohammad N SarboloukiHossain-Ali Rafiee-Pour
May 10, 2005·Proteomics·Erin J FinehoutKelvin H Lee

❮ Previous
Next ❯

Citations

Jun 28, 2012·Applied Biochemistry and Biotechnology·Petr PrikrylZdenka Kucerova
Dec 21, 2013·Journal of Proteome Research·Evgeniy V PetrotchenkoChristoph H Borchers
Feb 9, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Martina TylichováMarek Sebela
Feb 6, 2014·Chemical Reviews·María L VillalongaReynaldo Villalonga
Sep 3, 2010·Archaea : an International Microbiological Journal·Catherine H BottingMalcolm F White
Feb 15, 2013·The Journal of Biological Chemistry·David KopečnySolange Moréra
Jun 24, 2008·Journal of Molecular Biology·David KopecnýMichel Laloue
Dec 3, 2009·Proteomics·Jungbae KimRichard D Smith
Oct 16, 2007·Biochimica Et Biophysica Acta·Razvan D MicleaSathy V Balu-Iyer
Feb 12, 2008·Analytical Biochemistry·Tat'ána StosováZbynek Zdráhal
Jun 9, 2016·Biochimica Et Biophysica Acta·Jana BeinhauerMarek Šebela
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Feb 5, 2019·Biotechnology and Applied Biochemistry·Zdeněk PerutkaMarek Šebela
Oct 12, 2019·Journal of Mass Spectrometry : JMS·Zdeněk Perutka, Marek Šebela
Apr 12, 2013·Journal of Separation Science·Petr PrikrylZdenka Kucerova
Sep 28, 2007·Molecular Biology Reports·Katerina KolaríkováIvo Frébort
Mar 29, 2020·Journal of Experimental Botany·Ondřej KocábAndrej Pavlovič
Jul 4, 2008·Journal of Proteome Research·Melkamu Getie-KebtieMichail A Alterman
Feb 6, 2007·Journal of Chromatography. a·Benito CañasJose Manuel Gallardo
Sep 5, 2021·Journal of Proteomics·Maryam BaniasadMichael A Freitas
Sep 10, 2021·Frontiers in Plant Science·Zdeněk PerutkaMarek Šebela
Nov 5, 2021·Environmental Microbiology·Kateřina SnopkováDavid Šmajs

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.