Thermostable xylanase10B from Clostridium acetobutylicum ATCC824

Journal of Industrial Microbiology & Biotechnology
Mursheda K AliGeorge N Bennett

Abstract

The Clostridium acetobutylicum xylanase gene xyn10B (CAP0116) was cloned from the type strain ATCC 824, whose genome was recently sequenced. The nucleotide sequence of C. acetobutylicum xyn10B encodes a 318-amino acid protein. Xyn10B consists of a single catalytic domain that belongs to family 10 of glycosyl hydrolases. The enzyme was purified from recombinant Escherichia coli. The Xyn10B enzyme was highly active toward birchwood xylan, oat-spelt xylan, and moderately active toward avicel, carboxymethyl cellulose, polygalacturonic acid, lichenan, laminarin, barley-beta-glucan and various p-nitrophenyl monosaccharides. Xyn10B hydrolyzed xylan and xylooligosaccharides to produce xylobiose and xylotriose. The pH optimum of Xyn10B was 5.0, and the optimal temperature was 70 degrees C. The enzyme was stable at 60 degrees C at pH 5.0-6.5 for 1 h without substrate. This is one of a number of xylan-related activities encoded on the large plasmid in C. acetobutylicum ATCC 824.

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Citations

Dec 20, 2013·Environmental Technology·Bettina Schiel-BengelsdorfPeter Dürre
Nov 6, 2013·BMC Biotechnology·Fahimeh Salimi, Radhakrishnan Mahadevan
Jan 8, 2015·Applied Biochemistry and Biotechnology·Choong Hey NgKun-Lin Yang
Apr 9, 2013·Applied Biochemistry and Biotechnology·Carolina Cândida de Queiroz Brito-CunhaLuiz Artur Mendes Bataus

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