Thermotolerance and intracellular pH in two Chinese hamster cell lines adapted to growth at low pH

Journal of Cellular Physiology
M L WahlC S Owen

Abstract

As an in vitro model for the low extracellular pH (pHe) which has frequently been observed in tumors, cell lines have been grown in a low-pH medium in order to allow cell adaptation to that milieu. Two Chinese hamster cell lines [Chinese hamster ovary (CHO) and Chinese hamster ovarian carcinoma (OvCa)] were compared, both of which acquired thermotolerance during 42 degrees C heating in pHe = 7.3 buffer, but not in pHe = 6.7 medium unless grown at that pH long enough to become adapted. CHO cells, even when acutely acidified, showed higher intracellular pH (pHi) values in a suspension assay than OvCa cells, which confirmed the danger of comparing absolute values of pHi between cell lines. Despite this fundamental difference, relative changes in pHi were similar in that both lines showed a higher pHi in adapted than in unadapted cells, over the range of pHe values tested. The upregulation of pHi was statistically significant, but the two lines differed in the time frame over which adaptation occurred. OvCa cells acquired an enhanced ability to develop tolerance to 42 degrees heat at pHe = 6.7 in 4 days, but the CHO cells acquired this ability more progressively, achieving a maximum ability at approximately 100 days. In contrast, b...Continue Reading

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Citations

May 1, 1997·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·R A CossC S Owen
May 1, 1997·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·P R WachsbergerR A Coss
May 20, 1998·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·C S OwenD B Leeper
Oct 17, 2002·Endothelium : Journal of Endothelial Cell Research·Miriam L WahlDerrick S Grant
Dec 4, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Ronald Allen CossDennis B Leeper
May 27, 2004·Technology in Cancer Research & Treatment·Robert J GriffinJohn C Bischof
Nov 25, 1997·Journal of Cellular Physiology·C S OwenD B Leeper

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