Thermotolerance expression in mitotic CHO cells without increased translation of heat shock proteins

Journal of Cellular Physiology
M BorrelliP M Corry

Abstract

The objective of this study was to unequivocally demonstrate thermotolerance expression in mammalian cells in the absence of stress-induced synthesis of heat shock proteins (HSPs). Mitotic cells were selected as an experimental system since their genome was in the form of condensed chromosomes and ostensibly incapable of being transcribed; thus, obviating stress-induced HSP gene expression. Asynchronous Chinese hamster ovary (CHO) cells were treated with 0.2 microgram/ml nocodazole to accumulate cells in mitosis for harvest by mitotic shakeoff. Cells were maintained in mitosis with nocodazole during thermotolerance induction, thermotolerance development, and all challenge hyperthermia exposures. Although the heat shock transcription factor was activated by the thermotolerance inducing heat shock, as indicated by gel mobility shift assay, no increase in steady-state HSP mRNA levels was detected, as expected. Preferential synthesis of HSPs from extant mRNA was not detected during thermotolerance development and cellular levels of the 27 kDa, 70 kDa, and 90 kDa heat shock proteins remained constant, as determined by Western Blot analyses. The magnitude and induction threshold of expressed thermotolerance was not diminished when ce...Continue Reading

References

Aug 7, 1975·Nature·E W Gerner, M J Schneider
Sep 1, 1992·International Journal of Radiation Biology·J Laski, Z Jóźwiak
Apr 1, 1992·Journal of Cellular Physiology·S B BaderS K Calderwood
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G C LiW M Lee
Jul 1, 1991·European Journal of Biochemistry·C E AngelidisG N Pagoulatos
May 31, 1991·Biochemical and Biophysical Research Communications·M J BorrelliJ R Lepock
Jan 1, 1989·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M J BorrelliW C Dewey
Jul 1, 1989·The Journal of Cell Biology·J LandryL A Weber
Sep 1, 1988·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·A Laszlo
Jan 1, 1987·Biochemical Pharmacology·M L FreemanM J Meredith
Jan 1, 1987·Biochemical Pharmacology·J J Freeman, E P Hayes
Aug 1, 1982·The British Journal of Radiology·J R SubjeckR J Johnson
May 1, 1995·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M J BorrelliR A Coss
Jul 1, 1993·Journal of Cellular Physiology·M J BorrelliP M Corry
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R Y LiuG C Li

❮ Previous
Next ❯

Citations

Oct 13, 1998·In Vitro Cellular & Developmental Biology. Animal·C F Aréchiga, P J Hansen
Jun 3, 2005·Molecular Biology of the Cell·Henderika M J HutOdy C M Sibon
Dec 20, 2011·The Journal of Obstetrics and Gynaecology Research·Eunkuk ParkJong-Phil Chu
Sep 1, 2004·Biochemical and Biophysical Research Communications·Kyung-Cheol SohnSu-Il Do
May 14, 2009·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Andrei Laszlo, Ilona Fleischer
Aug 19, 2000·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·J Van RijnR Van Wijk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.