Theta-like activity in hippocampal formation slices: the effect of strong disinhibition of GABAA and GABAB receptors

Brain Research
J KonopackiR Grabowski

Abstract

The involvement of GABAA and GABAB receptors in neural mechanisms responsible for the production of theta rhythms in hippocampal formation (HPC) slices is addressed in the present study. In a number of papers published in the last decade, we have demonstrated that theta-like activity can be successfully recorded in the limbic cortex maintained in vitro when the cholinergic agonists, acetylcholine, carbachol or muscarine, were added to the bath. Recently, we have also shown a strong GABAA modulation of the cholinergic-induced in vitro theta-like activity. This study presents a report of the first demonstration of in vitro theta-like field responses induced a consequence of simultaneously inhibiting hippocampal GABAA and GABAB receptors. HPC slices (350 microns) were maintained in a gas-liquid interface chamber (35 degrees C). Theta-like activity was induced in the presence of bath perfusion of bicuculline (GABAA antagonist) and 2-hydroxysaclophen (GABAB antagonist). This in vitro induced field response was antagonized both by muscimol (GABAA agonist) and baclophen (GABAB agonist). In addition, the experiments presented here revealed that bicuculline/2-hydroxysaclophen-induced in vitro theta-like activity also had a strong cholin...Continue Reading

References

Jan 1, 1986·Progress in Neurobiology·B H Bland
Nov 1, 1980·Electroencephalography and Clinical Neurophysiology·T E Robinson, D J Green

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Citations

Oct 10, 2002·Brain Research Bulletin·Tomasz Kowalczyk, Jan Konopacki
Apr 22, 2014·Brain Research Bulletin·Jan KonopackiPaulina Kłos-Wojtczak
Apr 21, 2006·The European Journal of Neuroscience·Jan KonopackiHenryk Gołebiewski
Oct 23, 2012·International Journal of Experimental Pathology·Paulina KazmierskaJan Konopacki
Jun 27, 2012·Hippocampus·Tomasz KowalczykBartosz Caban
Jan 3, 2013·The European Journal of Neuroscience·Tomasz KowalczykJan Konopacki
Jul 31, 2013·Brain Research Bulletin·Paulina Kazmierska, Jan Konopacki
Aug 6, 2013·Brain Research·Alexandre Ademar HoellerThereza Christina Monteiro de Lima
Feb 7, 2013·Experimental Neurology·Valentina KitchiginaRubin Aliev

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