TheVibrio cholerae VexGH RND Efflux System Maintains Cellular Homeostasis by Effluxing Vibriobactin

MBio
Dillon E KunkleJames E Bina

Abstract

Resistance-nodulation-division (RND) superfamily efflux systems have been widely studied for their role in antibiotic resistance, but their native biological functions remain poorly understood. We previously showed that loss of RND-mediated efflux inVibrio choleraeresulted in activation of the Cpx two-component regulatory system, which mediates adaptation to stress resulting from misfolded membrane proteins. Here, we investigated the mechanism linking RND-mediated efflux to the Cpx response. We performed transposon mutagenesis screening of RND-deficientV. choleraeto identify Cpx suppressors. Suppressor mutations mapped to genes involved in the biosynthesis of the catechol siderophore vibriobactin. We subsequently demonstrated that vibriobactin secretion is impaired in mutants lacking the VexGH RND efflux system and that impaired vibriobactin secretion is responsible for Cpx system activation, suggesting that VexGH secretes vibriobactin. This conclusion was bolstered by results showing thatvexGHexpression is induced by iron limitation and thatvexH-deficient cells exhibit reduced fitness during growth under iron-limiting conditions. Our results support a model where VexGH contributes to cellular homeostasis by effluxing vibriobac...Continue Reading

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Citations

Sep 25, 2018·Environmental Microbiology Reports·Cara M DeAngelisJyl S Matson
Feb 20, 2020·Future Microbiology·Abigail L ColcloughJessica Ma Blair
Jan 9, 2019·Infection and Immunity·Mondraya F HowardJames E Bina
Dec 24, 2019·Frontiers in Microbiology·Randi L GuestTracy L Raivio
Nov 25, 2017·Microbiology and Immunology·Jesús A Pérez-AcostaJosé Ángel Huerta-Ocampo
Jul 14, 2018·Nature Reviews. Microbiology·Dijun DuBen F Luisi
Aug 10, 2017·Frontiers in Cellular and Infection Microbiology·Yingjie Li, Qingjun Ma
Sep 26, 2019·Frontiers in Cellular and Infection Microbiology·Claire L HewsTracy L Raivio
Aug 26, 2020·Journal of Bacteriology·Tanisha TeelucksinghGeorgina Cox

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Methods Mentioned

BETA
PCR
PCRs

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