Thiabendazole is an inducer of cytochrome P4501A1 in cultured rabbit hepatocytes

Biochemical and Biophysical Research Communications
L AixP Galtier

Abstract

The effect of TBZ (30-100 microM) was investigated on cytochromes P450 of cultured rabbit hepatocytes considered 72 h after plating. At the highest concentrations and without apparent cellular toxicity, the drug provokes a dose-dependent increase in total microsomal cytochrome P450 and a rise in EROD activity which was correlated to a specific increase in P4501A1 level. Northern blot analysis of RNA reveals an increased level of mRNA specific to P4501A1. The transcriptional activation of this isoenzyme is proposed because of the significant inhibition of the above-mentioned increases when actinomycin was added to the culture medium. Data obtained from competition experiments demonstrate that TBZ is not a ligand of Ah receptor. A down-regulation process could explain the slight decrease in both ANOH and P4502E1 level observed in hepatocytes treated with the highest dose of TBZ.

Citations

Dec 14, 2007·Chemical Research in Toxicology·Linh P Nguyen, Christopher A Bradfield
Jan 24, 2003·Annual Review of Pharmacology and Toxicology·Michael S Denison, Scott R Nagy
Aug 23, 2006·Expert Opinion on Drug Metabolism & Toxicology·Preeti RamadossGary H Perdew
Jan 10, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Marilyne SéïdeDiana A Averill-Bates
Jun 24, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R J PriceB G Lake
Jul 1, 1996·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·X Rey-GrobelletP Galtier
Jun 10, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Rob StierumJohn Groten
Sep 6, 2015·Biochemical Pharmacology·Chiara RedaelliTobias Volker Lanz
Jun 1, 2005·Research in Veterinary Science·M SavlíkL Skálová
Dec 16, 1998·Toxicology and Applied Pharmacology·M S DenisonM H Ziccardi
Mar 29, 2014·Cytotechnology·Hideo SatsuMakoto Shimizu
Mar 15, 2000·Regulatory Toxicology and Pharmacology : RTP·J P GrotenR Walker
Oct 4, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Waranya ChatuphonprasertKanokwan Jarukamjorn
Jun 6, 2016·Ecotoxicology and Environmental Safety·Diana MaierRita Triebskorn
Apr 28, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Roger J PriceBrian G Lake
Apr 8, 2018·Mucosal Immunology·Bruno LamasHarry Sokol
Mar 7, 2007·Archives of Toxicology·Yasuaki DewaKunitoshi Mitsumori
Aug 30, 2008·Archives of Toxicology·Yasuaki DewaKunitoshi Mitsumori
Mar 21, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Roslyn S ThelingwaniCollen M Masimirembwa
Feb 12, 1998·The Journal of Biological Chemistry·M BacklundM Ingelman-Sundberg
Jul 26, 1996·The Journal of Biological Chemistry·M D SadarT B Andersson
Apr 14, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Kevin J CoeJ Greg Slatter
Jan 6, 2000·Archives of Biochemistry and Biophysics·C SandozA Carpy
Nov 25, 2000·Toxicology·C DelescluseR Rahmani
Apr 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C Gleizes-EscalaP Galtier

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