Thiamin deficiency induces impaired fish gill immune responses, tight junction protein expression and antioxidant capacity: Roles of the NF-κB, TOR, p38 MAPK and Nrf2 signaling molecules

Fish & Shellfish Immunology
Ling-Mei WenXiao-Qiu Zhou

Abstract

In this study, we investigate the effects of dietary thiamin deficiency on immune responses, tight junctions, antioxidant capacity and related signaling molecules in the gills of young grass carp (Ctenopharyngodon idella). Fish were fed diets that contained 0.12-2.04 mg thiamin kg(-1) for 8 weeks. We found that dietary thiamin deficiency resulted in reduced complement 3 content, lysozyme and acid phosphatase activities, mRNA levels of hepcidin, liver-expressed antimicrobial peptides 2, transforming growth factor (TGF)-β1, interleukin (IL)-10, inhibitor protein-κBα (IκBα), ribosomal S6 protein kinase 1 and target of rapamycin (TOR) and increased expression of interferon-γ2, tumor necrosis factor-α, TGF-β2, IL-1β, IL-8, IκB kinases (IKKβ and IKKγ) and nuclear factor-κB p65 (NF-κB p65). Our findings showed that thiamin deficiency reduced the immune status of fish gills. Furthermore, thiamin deficiency resulted in reduced mRNA transcript levels of claudin b, claudin 3, claudin 12, zonula occludens 1 (ZO-1) and occludin and increased mRNA transcript levels of claudin 15a, myosin light-chain kinase (MLCK) and p38 mitogen-activated protein kinase (p38 MAPK) in fish gill tissues. These data suggested that thiamin deficiency disrupted t...Continue Reading

References

May 1, 1984·Journal of Neuropathology and Experimental Neurology·H AikawaT Moroji
Oct 14, 2004·Cell·Dongsheng CaiSteven E Shoelson
May 24, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Carlos BarataCinta Porte
May 27, 2005·Fish & Shellfish Immunology·B MagnadottirR A Dalmo
Jun 30, 2006·Cellular Signalling·Laura CallerosAntonio Chiloeches
Dec 8, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Victoria J Berdikova BohneAugustine Arukwe
May 15, 2007·Developmental and Comparative Immunology·Pinwen Peter ChiouThomas T Chen
Oct 24, 2007·Biochimica Et Biophysica Acta·Lorenza González-MariscalDavid Chamorro
Nov 28, 2007·The Journal of Experimental Medicine·Mike G LaukoetterCharles A Parkos
Oct 14, 2008·Immunity·Thomas WeichhartMarcus D Säemann
Jul 7, 2009·Neurochemistry International·Rossana C ZepedaArturo Ortega
Oct 1, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M D SäemannT Weichhart
May 24, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yusuke KumaiSteve F Perry
May 30, 2012·Respiratory Physiology & Neurobiology·Helen ChasiotisScott P Kelly
Jul 21, 2012·General and Comparative Endocrinology·Kannikar Wongdee, Narattaphol Charoenphandhu
Apr 17, 2013·Physiological Reviews·Dorothee Günzel, Alan S L Yu
Sep 17, 2013·Fish & Shellfish Immunology·Goshi KatoChihaya Nakayasu
Sep 17, 2013·Fish & Shellfish Immunology·Tiehui Wang, Christopher J Secombes
Sep 21, 2013·Digestive Diseases and Sciences·Lily NahidiAndrew S Day
Oct 9, 2013·Fish & Shellfish Immunology·Daniela GomezIrene Salinas
May 6, 2014·Fish & Shellfish Immunology·Shuang LiangCaihuan Ke
May 6, 2014·Fish & Shellfish Immunology·Carlo C Lazado, Christopher Marlowe A Caipang
Jun 14, 2014·Ecotoxicology and Environmental Safety·Erica GiarratanoGabriela Malanga

❮ Previous
Next ❯

Citations

Jul 30, 2020·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Elaheh AmiraniAlireza Abed
Feb 23, 2020·Environmental Science and Pollution Research International·Yimeng WangXiangping Nie
Aug 25, 2020·Journal of Neuro-ophthalmology : the Official Journal of the North American Neuro-Ophthalmology Society·Maxwell Q NyceJorge C Kattah

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.