Thiamin pyrimidine biosynthesis in Candida albicans : a remarkable reaction between histidine and pyridoxal phosphate.

Journal of the American Chemical Society
Rung-Yi LaiTadhg P Begley

Abstract

In Saccharomyces cerevisiae , thiamin pyrimidine is formed from histidine and pyridoxal phosphate (PLP). The origin of all of the pyrimidine atoms has been previously determined using labeling studies and suggests that the pyrimidine is formed using remarkable chemistry that is without chemical or biochemical precedent. Here we report the overexpression of the closely related Candida albicans pyrimidine synthase (THI5p) and the reconstitution and preliminary characterization of the enzymatic activity. A structure of the C. albicans THI5p shows PLP bound at the active site via an imine with Lys62 and His66 in close proximity to the PLP. Our data suggest that His66 of the THI5 protein is the histidine source for pyrimidine formation and that the pyrimidine synthase is a single-turnover enzyme.

References

Apr 25, 2002·Journal of the American Chemical Society·Johannes ZeidlerIan D Spenser
Mar 4, 2008·Current Opinion in Chemical Biology·Tadhg P BegleySteven E Ealick
Apr 5, 2008·Journal of Nutritional Science and Vitaminology·Shiho IshidaKazuko Yamada
Apr 8, 2009·Annual Review of Biochemistry·Christopher T JurgensonSteven E Ealick
Oct 28, 2011·Nature·Abhishek ChatterjeeTadhg P Begley

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Citations

Oct 29, 2013·Journal of Structural Biology·Sandrine CoquilleStéphane Thore
Sep 27, 2014·Current Opinion in Structural Biology·Teresa B Fitzpatrick, Stéphane Thore
Aug 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Michael MoulinTeresa B Fitzpatrick
Aug 20, 2014·Frontiers in Plant Science·Jiahn-Chou GuanDonald R McCarty
Jun 7, 2016·Journal of Nutritional Science and Vitaminology·Yukie KijimaKeiko Tazuya-Murayama
Jan 28, 2017·Journal of the American Chemical Society·Samantha L PilicerChristian Wolf
Jan 24, 2020·Yeast·Thomas PerliJean-Marc Daran
Feb 12, 2020·Nature Chemical Biology·Kei SakakiMakoto Nishiyama
Oct 10, 2020·Molecular Microbiology·Michael D PaxhiaDiana M Downs
Jun 17, 2018·Plant Science : an International Journal of Experimental Plant Biology·Andrew D HansonYousong Ding
Dec 1, 2018·G3 : Genes - Genomes - Genetics·Michael D Paxhia, Diana M Downs
Mar 25, 2014·Biochemistry·Lisa E CooperTadhg P Begley

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