Jan 1, 1975

Thiamine triphosphatase activity of myosin and accelerating effect of thiamine di- and tri-phosphates on superprecipitation of actomyosin

Journal of Nutritional Science and Vitaminology
A Murai, E Katsura

Abstract

TTP accelerated ATP-induced superprecipitation of actomyosin in as low a concentration as 30 muM and decreased light scattering by actomyosin. These effects could also be observed in the same way, but to a lesser degree, by addition to TDP. Myosin was able to hydrolyze TTP to TDP, but some important differences were confirmed between myosin TTPase and ATPase. Myosin TTPase was inhibited by actin and showed a much larger Km than that of ATPase. TTP significantly inhibited myosin B ATPase and ATP greatly inhibited myosin B TTPase. These findings suggest that the accelerating effect of TDP and TTP may be due to the binding of thiamine phosphate to the regulatory site of myosin followed by a change in its physical chemical property, rather than due to the competitive binding of thiamine phosphate to the catalytically activity site of myosin.

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Mentioned in this Paper

Phosphoric Monoester Hydrolases
Thiamine Mononitrate
Berolase
Adenosine Triphosphatases
Isoactin
Actins
Actomyosin Location
Metals
Thiamine Assay
MYH14

About this Paper

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