Thiazolidinediones stimulate uncoupling protein-2 expression in cell lines representing white and brown adipose tissues and skeletal muscle

Endocrinology
A CamirandJ E Silva

Abstract

Thiazolidinediones (TZD) are PPAR gamma ligands that sensitize tissues to insulin. A cDNA encoding a mitochondrial protein likely to act as uncoupler (uncoupling protein 2, UCP2) has been recently cloned. Since TZD have been reported to increase energy expenditure in animals, we have examined the effects of these drugs on the expression of UCP2 mRNA in cell lines representing white (3T3-L1 and 3T3-F442A) and brown (HIB-1B) adipose tissues and skeletal muscle (L6). Northern blots probed with a mouse UCP2 full-length cDNA showed a mRNA of 1.6 kb both in tissues and the aforementioned cells lines. Within 4 h of exposing these cells to 30 microM darglitazone, there was an increase in UCP2 mRNA which reached a plateau of 5-10 times the basal in about 8 h. In all cells TZDs (darglitazone, troglitazone) were more active than the predominantly PPAR alpha ligands WY-14,613 and clofibrate, or the non-selective ligand linoleic acid. These results indicate that TZDs can stimulate the expression of UCP2 gene probably via PPAR gamma and hence have the potential to increase energy expenditure in adult humans, in whom UCP2 is expressed ubiquitously.

Citations

Jan 6, 2000·The Biochemical Journal·D Ricquier, F Bouillaud
Sep 12, 2012·PPAR Research·Gene P Ables
Jan 8, 2008·World Journal of Gastroenterology : WJG·Eric R KallwitzScott J Cotler
Nov 4, 2000·Biochemical and Biophysical Research Communications·J M Reilly, M P Thompson
Jun 30, 2000·Biochemical and Biophysical Research Communications·T TeruelJ C Clapham
Oct 27, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daozhong JinXiaoli Chen
Feb 24, 2006·Toxicology and Applied Pharmacology·Anabela P Rolo, Carlos M Palmeira
Dec 1, 2009·IUBMB Life·Tomo YonezawaHidetoshi Inoko
Feb 23, 2013·Molecular and Cellular Endocrinology·Anna Pereira-FernandesRonny Blust
Mar 18, 2004·British Journal of Cancer·P M Sanders, M J Tisdale
Jun 16, 2004·FEBS Letters·Mary P Thompson, Dongho Kim
Jul 31, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·U RisérusB Vessby
Mar 13, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·A MartiJ A Martinez
Dec 26, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·Y Hatakeyama, P J Scarpace
Jun 13, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·J E DigbyS O'Rahilly
Jun 1, 2001·Journal of Endocrinological Investigation·R Perfetti, K Chamie
Feb 19, 1999·Obesity Research·P SchrauwenE Ravussin
Dec 29, 2015·Molecular and Cellular Biochemistry·Mervat Y HanafiMaher A Kamel
Feb 8, 2020·Journal of Cellular and Molecular Medicine·Liwei WuJianye Wu
Aug 24, 1999·Endocrine Reviews·L PoretskyL C Giudice
Jul 27, 2000·American Journal of Physiology. Endocrinology and Metabolism·X X YuS H Adams
Dec 18, 2001·Experimental Biology and Medicine·S CollinsR Surwit
Nov 10, 2001·American Journal of Physiology. Endocrinology and Metabolism·M B Armstrong, H C Towle
Mar 4, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K A Van Der LeeM Van Bilsen
Jun 29, 2013·Cellular and Molecular Life Sciences : CMLS·Massimo DonadelliMarta Palmieri
Apr 19, 2002·Journal of Applied Physiology·George Argyropoulos, Mary-Ellen Harper
Nov 24, 1999·Biochemical and Biophysical Research Communications·N TuK U Lentes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.