Thin-layer chromatographic analysis of cocaine and benzoylecgonine in urine

Journal of Chromatography
J E WallaceK Blum

Abstract

The sensitivity achieved by the described thin-layer chromatographic (TLC) method greatly exceeds that of previously published TLC methods for the determination of cocaine and its principal metabolite, benzoylecgonine, in urine. Sensitivity for cocaine and benzoylecgonine approaches 0.1 and 0.25 mug/ml, respectively, for a 5.0-ml specimen. A simple extraction with a mixed organic solvent provides the basic mechanism for isolating the drugs from biologic specimens. Cocaine and its metabolites are stable in sulfuric acid solutions but labile in aqueous media containing certain other inorganic and organic acids; therefore, an emphasis on the utilization of sulfuric acid solutions is employed throughout the procedure. An evaluation of sensitivities achieved for cocaine and benzoylecgonine by various detection reagents is presented. The technique is applicable to drug screening programs.

Citations

Mar 1, 1981·Journal of Ethnopharmacology·J E Lindgren
Mar 14, 2016·Forensic Science International : Synergy·Thays C De CarvalhoBoniek G Vaz
Nov 1, 1984·Forensic Science International : Synergy·K MatsubaraY Fukui
Mar 7, 2006·Legal Medicine·Mauricio Yonamine, Mônica Cortez Sampaio
Jul 1, 1981·Clinical Toxicology·R D Budd
Mar 1, 1980·Clinical Toxicology·R D BuddF C Yang
May 1, 1977·The Annals of Otology, Rhinology, and Laryngology·M E JohnsR L Henderson
Mar 1, 1995·Annals of Clinical Biochemistry·R A BraithwaiteB Widdop

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