PMID: 45617Jan 1, 1977

Thin layer chromatographic extraction of the histologic section--a combination of microanalytic and histochemical methods

Acta histochemica. Supplementband
M Inczedy-MarcsekG Vatterodt

Abstract

Thin layer chromatography as extraction method for histological sections. A combined microanalytical and histochemical procedure. A combined histochemical and chromatographical procedure is described to identify tissue components such as lipids, free amino acids, carbohydrates and steroids in cryosections. Fixed or unfixed sections are extracted by solvents of decreasing or increasing polarity. The extracts are then analysed by chromatographical techniques known from analysis of tissue homogenates. The extracted sections can be controlled by histochemical staining. Our technique is demonstrated in detail by analysis of lipids in bovine seminal vesicle epithelium, by analysis of free-amino acids in rat brain and pituitary and by analysis of steroids in male rat adrenal gland.

Related Concepts

Carbohydrate nutrients
Amino Acids, I.V. solution additive
Pituitary Diseases
Bos taurus
Chromatography, Qualitative; Thin Layer, Analyte Not Elsewhere Specified
Catatoxic Steroids
Adrenal Glands
Brain Chemistry
Pituitary Hormonal Agents
Rat Adrenal Gland

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