Thin-layer chromatography combined with diode laser desorption/atmospheric pressure chemical ionization mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Song PengJoachim Franzke

Abstract

The desorption of an analyte by a continuous wave diode laser from a porous surface of a thin-layer plate covered with a graphite suspension is presented. The thermally desorbed analyte molecules are ionized in the gas phase by a corona discharge at atmospheric pressure. Therefore, both essential processes--the desorption and the ionization of analyte molecules, which are often performed in one step--are separated. The target preparation is easy and fast since no additional extraction process is required. The mass spectrometric background signal was mostly limited to the low mass range showing no interference with typical compounds of interest. In this study, the calmative and antihypertensive drug reserpine was chosen as model analyte, which is often used for specification of mass spectrometers. No fragmentation was observed because of efficient collisional cooling under atmospheric pressure. The influence of diode laser power and the composition of the graphite suspension were investigated, and a primary optimization was performed.

Citations

Feb 22, 2011·Journal of Chromatography. a·Sy-Chyi ChengJentaie Shiea
Nov 9, 2004·Journal of Mass Spectrometry : JMS
Jul 22, 2010·Journal of Mass Spectrometry : JMS·Lee Chuin ChenKenzo Hiraoka
Feb 20, 2013·Analytical Chemistry·Bienvenida Gilbert-LópezJoachim Franzke
May 27, 2021·Mass Spectrometry Reviews·Maria Del Mar Boronat EnaVincenzo Abbate
Jun 16, 2021·Mass Spectrometry Reviews·Hanlu YueYixiang Duan

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