PMID: 13549493May 25, 1958Paper

Thin sections. I. A study of section thickness and physical distortion produced during microtomy

The Journal of Biophysical and Biochemical Cytology
L D PEACHEY

Abstract

Knowledge of the thickness of sections is important for proper interpretation of electron micrographs. Therefore, the thicknesses of sections of n-butyl methacrylate polymer were determined by ellipsometry, and correlated with the color shown in reflected light. The results are: gray, thinner than 60 mmicro; silver, 60 to 90 mmicro; gold, 90 to 150 mmicro; purple, 150 to 190 mmicro; blue, 190 to 240 mmicro; green, 240 to 280 mmicro; and yellow, 280 to 320 mmicro. These results agree well with optical theory and with previous published data for thin films. Sections, after cutting, are 30 to 40 per cent shorter than the face of the block from which they were cut. Only a small improvement results from allowing the sections to remain in the collecting trough at room temperature. Heating above room temperature, however, reduces this shortening, with a corresponding improvement in dimensions and spatial relationships in the sections. When the thickness of the section is considered in interpreting electron micrographs instead of considering the section to be two-dimensional, a more accurate interpretation is possible. The consideration of electron micrographs as arising from projections of many profiles from throughout the whole thick...Continue Reading

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Citations

Jan 1, 1966·Zeitschrift für Zellforschung und mikroskopische Anatomie·F ClementiM N Sheridan
Jun 1, 1988·Journal of Neurocytology·J DeFelipe, A Fairén
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Dec 1, 1961·The Journal of Biophysical and Biochemical Cytology·A F BIRD
Nov 1, 1962·The Journal of Cell Biology·L G CARO
Jan 1, 1964·The Journal of Cell Biology·B BOOTHROYD
Jun 1, 1968·The Journal of Cell Biology·G E Palade, R R Bruns
May 25, 1958·The Journal of Biophysical and Biochemical Cytology·P G SATIR, L D PEACHEY
Mar 1, 1969·The Journal of Cell Biology·L SilvermanD Glick
Apr 1, 1969·The Journal of Cell Biology·M M SalpeterE E Salpeter
Aug 1, 1969·The Journal of Cell Biology·R R Bruns
Jun 1, 1971·The Journal of Cell Biology·C E Devine, A P Somlyo
Dec 1, 1971·The Journal of Cell Biology·N B Gilula, P Satir
Mar 1, 1972·The Journal of Cell Biology·N YagoH Kurokawa

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