Thioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils

Scientific Reports
Anna I SulatskayaKonstantin K Turoverov

Abstract

Fluorescence of thioflavin T (ThT) is a proven tool for amyloid fibrils study. The correct model of ThT binding to fibrils is crucial to clarify amyloid fibrils structure and mechanism of their formation. Although there are convincing evidences that ThT has molecular rotor nature, implying it's binding to fibrils in monomer form, speculations concerning ThT binding to fibrils in aggregated forms appear in literature so far. The elaborated approach for fluorescence intensity correction on the inner filter effects applied to ThT aqueous solutions with a wide range of concentration allowed characterizing ThT excimers fluorescence and showing its difference from that of ThT bound to fibrils. Obtained results experimentally prove the monomer model of ThT binding to amyloid fibrils and demonstrate wide capacity of the used approach in the spectroscopy of other fluorescent dyes for examination of concentration self-quenching and deformation of fluorescence spectra, dye molecules interaction, dimers and excimers formation.

References

Sep 2, 1999·Trends in Biochemical Sciences·C M Dobson
Mar 8, 2003·Methods in Enzymology·David M JamesonPierre D J Moens
Dec 20, 2003·Nature·Christopher M Dobson
Jan 5, 2005·Journal of Structural Biology·M R H KrebsA M Donald
Aug 30, 2005·Journal of Structural Biology·Ritu KhuranaShashi Singh
Nov 24, 2005·The FEBS Journal·Thomas R Jahn, Sheena E Radford
Sep 7, 2006·Journal of Computational Chemistry·Stefan Grimme
Feb 14, 2007·Macromolecular Bioscience·Ute SlottaThomas Scheibel
Feb 20, 2007·Journal of Proteome Research·Alexander A MaskevichKonstantin K Turoverov
May 15, 2007·The Journal of Physical Chemistry. a·Vitali I StsiapuraIrina M Kuznetsova
Apr 16, 2009·The Journal of Physical Chemistry. B·Vitali I StsiapuraKonstantin K Turoverov
Aug 12, 2009·The Journal of Physical Chemistry. B·Catherine C Kitts, David A Vanden Bout
Apr 13, 2010·The Journal of Physical Chemistry. B·Prabhat K SinghSukhendu Nath
May 25, 2010·Chemico-biological Interactions·Jeffrey T AulettaTerrone L Rosenberry
Sep 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Leslie S WolfeYong Xiong
Mar 9, 2011·The Journal of Physical Chemistry. a·Nadav AmdurskyDan Huppert
May 19, 2011·The Journal of Physical Chemistry. a·Nadav AmdurskyDan Huppert
Jun 30, 2011·The Journal of Physical Chemistry. a·Yuval ErezDan Huppert
Aug 26, 2011·The Journal of Physical Chemistry. B·Anna I SulatskayaKonstantin K Turoverov
May 18, 2012·Molecular Neurobiology·Irina M KuznetsovaKonstantin K Turoverov
Jun 29, 2012·Accounts of Chemical Research·Nadav AmdurskyDan Huppert
Jul 5, 2012·Methods in Molecular Biology·Anna I SulatskayaKonstantin K Turoverov
May 21, 2013·Chemical Communications : Chem Comm·Raimon SabateSalvador Ventura
Jul 30, 2014·PloS One·Alexander V FoninKonstantin K Turoverov
Dec 8, 2015·Analytical Chemistry·Irina M KuznetsovaKonstantin K Turoverov

❮ Previous
Next ❯

Citations

Apr 28, 2018·Chemistry : a European Journal·Seung-Chul LeeO-Pil Kwon
Jan 22, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Suraj Kumar Panigrahi, Ashok Kumar Mishra
Mar 1, 2019·Scientific Reports·Martin J PageEtheresia Pretorius
Nov 7, 2019·Biomolecules·Anastasiia O KosolapovaAnton A Nizhnikov
Mar 18, 2020·Microscopy Research and Technique·Giuseppe SancataldoValeria Vetri
Jan 8, 2020·Chemistry : a European Journal·Jocky C K KungRebecca A Jockusch
Jul 24, 2020·PLoS Biology·Kirill S AntonetsAnton A Nizhnikov
Sep 12, 2019·Frontiers in Aging Neuroscience·Büin AdamsEtheresia Pretorius
Aug 26, 2018·International Journal of Molecular Sciences·Anna I SulatskayaKonstantin K Turoverov
Mar 2, 2021·The Analyst·Catarina S H JesusCarlos Serpa
Jan 24, 2019·Analytical Chemistry·A I SulatskayaK K Turoverov
Aug 29, 2019·ACS Omega·Mouli KonarSwagata Dasgupta

❮ Previous
Next ❯

Methods Mentioned

BETA
protein folding

Software Mentioned

FireFly
Cary Eclipse
SigmaPlot

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.