Thioflavin T specifically brightening "Guanine Island" in duplex-DNA: a novel fluorescent probe for single-nucleotide mutation

The Analyst
Wei ZhouXiaohong Li

Abstract

Here, we found that Thioflavin T (ThT) could specifically bind with a G-GGG unit (named as "Guanine Island") in double stranded DNA (ds-DNA). Through stacking with G base via hydrogen bonds, the rotation of ThT was restricted and concurrently its planarization was enforced. Such a binding mode produced a significantly enhanced ThT fluorescence. Based on this discovery, with ThT as a lighting-up probe for "Guanine Island" in ds-DNA, the fluorescent detection of single-nucleotide mutation (C mutation) was investigated. With C base in target DNA mutating to G, A or T and further hybridizing with a probe DNA containing a GGG unit at the mutated point, ds-DNA including G-GGG, A-GGG or T-GGG islands was formed, respectively. After binding with ThT, C mutation was clearly recognized. With C mutating to G as an example, the detection limit was as low as 3 nM. Importantly, the developed assay could be applied to recognize C mutating to G in a DNA sequence related to dilated cardiomyopathy for diluted human serum. As a sensing unit ("Guanine Island" in ds-DNA lighting up ThT), it is expected to be applied for various biological or environmental systems.

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Citations

Nov 8, 2020·Nanomaterials·Marcel HankeAdrian Keller
Jun 13, 2021·Biochimie·Smita VermaNihar Ranjan
Mar 7, 2020·The Journal of Physical Chemistry Letters·Srikrishna PramanikSaptarshi Mukherjee

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Methods Mentioned

BETA
biosensors
Fluorescence
Circular Dichroism
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