Thiol-Disulfide Exchange Reaction for Cellular Glutathione Detection with Surface-Enhanced Raman Scattering

Analytical Chemistry
Chenghua WeiHaifeng Yang

Abstract

Abnormal glutathione (GSH) levels are related to several diseases, including cancer. In this paper, surface-enhanced Raman spectroscopy (SERS) based on a 2-(pyridyldithio)ethylamine (PDEA)-modified AgNPs@Si wafer is proposed for intracellular GSH detection. PDEA plays multifunctional roles in the method, including the participation in the thiol-disulfide exchange reaction and the contribution of a great SERS signal reporter. With the addition of GSH, the disulfide bond of PDEA will be broken, releasing the pyridine ring in PDEA and resulting in a signal-off SERS response. The developed specific reaction-based SERS assay can detect GSH levels as low as 2.5 × 10-7 M. Subsequently, we employed this method to evaluate cellular GSH levels and found that the levels in cancer cells are higher than those in normal cells. The PDEA-modified AgNPs@Si-based SERS protocol demonstrates good selectivity and high sensitivity, as well as robustness, which is suitable to evaluate cellular GSH levels.

References

Jan 1, 1976·Annual Review of Biochemistry·A Meister, S S Tate
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·L A HerzenbergL A Herzenberg
Aug 10, 2000·European Journal of Biochemistry·J B SchulzJ Dichgans
Oct 29, 2000·Journal of Agricultural and Food Chemistry·A C AltingR W Visschers
Feb 15, 2002·The New England Journal of Medicine·Sudha SeshadriPhilip A Wolf
Jun 24, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Danyelle M TownsendHaim Tapiero
Feb 28, 2004·The Journal of Nutrition·Guoyao WuNancy D Turner
Jul 8, 2008·Molecular Aspects of Medicine·Shelly C Lu
Oct 1, 2010·Advanced Materials·Sonja BauhuberAchim Göpferich
Jul 27, 2011·ACS Applied Materials & Interfaces·Jia ZhangXiurong Yang
Mar 31, 2012·The Analyst·John C HarfieldRichard G Compton
Apr 19, 2015·Biosensors & Bioelectronics·Chunyang ChenWeisheng Liu
Jun 24, 2017·Chemical Society Reviews·M Fernanda CardinalRichard P Van Duyne
Jul 14, 2017·Angewandte Chemie·Cai-Xia YinRobert M Strongin

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Citations

Oct 3, 2020·The Analyst·Gregory Q Wallace, Jean-François Masson
Dec 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yue SunXiaowei Cao
Apr 13, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Fuming SangZhizhou Zhang
Apr 29, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qianru MaYufen Zhao
Jul 13, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Cailing JiangLi Jiang

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