Thiol modulation of the chloroplast ATP synthase is dependent on the energization of thylakoid membranes

Plant & Cell Physiology
Hiroki KonnoToru Hisabori

Abstract

Thiol modulation of the chloroplast ATP synthase γ subunit has been recognized as an important regulatory system for the activation of ATP hydrolysis activity, although the physiological significance of this regulation system remains poorly characterized. Since the membrane potential required by this enzyme to initiate ATP synthesis for the reduced enzyme is lower than that needed for the oxidized form, reduction of this enzyme was interpreted as effective regulation for efficient photophosphorylation. However, no concrete evidence has been obtained to date relating to the timing and mode of chloroplast ATP synthase reduction and oxidation in green plants. In this study, thorough analysis of the redox state of regulatory cysteines of the chloroplast ATP synthase γ subunit in intact chloroplasts and leaves shows that thiol modulation of this enzyme is pivotal in prohibiting futile ATP hydrolysis activity in the dark. However, the physiological importance of efficient ATP synthesis driven by the reduced enzyme in the light could not be demonstrated. In addition, we investigated the significance of the electrochemical proton gradient in reducing the γ subunit by the reduced form of thioredoxin in chloroplasts, providing strong ins...Continue Reading

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Citations

Nov 14, 2012·Antioxidants & Redox Signaling·Toru HisaboriHiroki Konno
Jun 25, 2014·Antioxidants & Redox Signaling·Peter Geigenberger, Alisdair R Fernie
Mar 15, 2013·The Journal of Biological Chemistry·Kaori KohzumaDavid M Kramer
Jun 2, 2016·Nature Plants·Hiroshi YamamotoToshiharu Shikanai
Oct 16, 2015·The Plant Journal : for Cell and Molecular Biology·Yuki Okegawa, Ken Motohashi
Oct 24, 2015·Protein Expression and Purification·Yuki OkegawaKen Motohashi
Dec 8, 2014·Biochimica Et Biophysica Acta·Caijuan WangToshiharu Shikanai
Apr 4, 2015·Annual Review of Biochemistry·Wolfgang Junge, Nathan Nelson
Jun 10, 2016·Environmental Science and Pollution Research International·Jingwen SunXiaohua Huang
Feb 3, 2016·Plant, Cell & Environment·Lauri NikkanenEevi Rintamäki
May 10, 2019·The Journal of Biological Chemistry·Kosuke InabeToru Hisabori
Dec 12, 2019·Frontiers in Plant Science·Carmen SiebenallerDirk Schneider
Dec 12, 2019·Frontiers in Plant Science·Keisuke YoshidaToru Hisabori
Aug 15, 2018·Proceedings of the National Academy of Sciences of the United States of America·Keisuke YoshidaToru Hisabori
Jul 14, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Takatoshi SekiguchiToru Hisabori
May 2, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Felix BuchertPierre Joliot

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