Thiophosphate induces apoptosis in human leukemia cell lines

Biochemical and Biophysical Research Communications
L JiangA D Conigrave

Abstract

Thiophosphate induced apoptosis in human HL-60 cells. HL-60 cell proliferation was inhibited with an IC50 of about 60 microM. Typical morphological changes of apoptosis were observed by phase contrast microscopy and DNA laddering was observed after agarose gel electrophoresis. Thiophosphate-induced DNA fragmentation was time and concentration-dependent. After exposure to thiophosphate (100 microM) apoptosis occurred as early as 4 h after treatment and 90% of cells were apoptotic by 24 h. dbcAMP-differentiated HL-60 cells as well as undifferentiated HL-60 cells were susceptible. Thiophosphate was also effective in inducing apoptosis in other leukemia cell lines including CEM and K562 and a lymphoma cell line, Raji.

Citations

Mar 17, 2016·Molecular and Cellular Biochemistry·Elizabeth G Frayne
Mar 27, 1997·Biochemical and Biophysical Research Communications·L JiangA D Conigrave

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis