Thioredoxin-1 (Trx1) engineered mesenchymal stem cell therapy increased pro-angiogenic factors, reduced fibrosis and improved heart function in the infarcted rat myocardium

International Journal of Cardiology
Sumanth C SureshNilanjana Maulik

Abstract

Engraftment of mesenchymal stem cells (MSCs) has emerged as a powerful candidate for mediating myocardial repair. In this study, we genetically modified MSCs with an adenovector encoding thioredoxin-1 (Ad.Trx1). Trx1 has been described as a growth regulator, a transcription factor regulator, a cofactor, and a powerful antioxidant. We explored whether engineered MSCs, when transplanted, are capable of improving cardiac function and angiogenesis in a rat model of myocardial infarction (MI). Rat MSCs were cultured and divided into MSC, MSC+Ad.LacZ, and MSC+Ad.Trx1 groups. The cells were assayed for proliferation, and differentiation potential. In addition, rats were divided into control-sham (CS), control-MI (CMI), MSC+Ad.LacZ-MI (MLZMI), and MSC+Ad.Trx1-MI (MTrxMI) groups. MI was induced by left anterior descending coronary artery (LAD) ligation, and MSCs preconditioned with either Ad.LacZ or Ad.Trx1 were immediately administered to four sites in the peri-infarct zone. The MSC+Ad.Trx1 cells increased the proliferation capacity and maintained pluripotency, allowing them to divide into cardiomyocytes, smooth muscle, and endothelial cells. Western blot analysis, 4 days after treatment showed increased vascular endothelial growth fac...Continue Reading

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Citations

Mar 26, 2016·Differentiation; Research in Biological Diversity·Sami G Almalki, Devendra K Agrawal
Apr 12, 2016·International Journal of Cardiology·Jichun WangHong Jiang
Jul 9, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Natalia VoroninaRobert David
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Feb 14, 2021·Journal of Hematology & Oncology·Tian ZhouPeilong Lai
Mar 5, 2021·Stem Cells Translational Medicine·Raquel Cruz-SamperioAdam Perriman
May 25, 2021·Bioengineering & Translational Medicine·Lily Li-Wen WangSamir Mitragotri
Sep 10, 2018·ACS Biomaterials Science & Engineering·Bonda Rama RaoChitta Ranjan Patra

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