Abstract
Two different thiol redox systems exist in plant chloroplasts, the ferredoxin-thioredoxin (Trx) system, which depends on ferredoxin reduced by the photosynthetic electron transport chain and, thus, on light, and the NADPH-dependent Trx reductase C (NTRC) system, which relies on NADPH and thus may be linked to sugar metabolism in the dark. Previous studies suggested, therefore, that the two different systems may have different functions in plants. We now report that there is a previously unrecognized functional redundancy of Trx f1 and NTRC in regulating photosynthetic metabolism and growth. In Arabidopsis (Arabidopsis thaliana) mutants, combined, but not single, deficiencies of Trx f1 and NTRC led to severe growth inhibition and perturbed light acclimation, accompanied by strong impairments of Calvin-Benson cycle activity and starch accumulation. Light activation of key enzymes of these pathways, fructose-1,6-bisphosphatase and ADP-glucose pyrophosphorylase, was almost completely abolished. The subsequent increase in NADPH-NADP(+) and ATP-ADP ratios led to increased nitrogen assimilation, NADP-malate dehydrogenase activation, and light vulnerability of photosystem I core proteins. In an additional approach, reporter studies sho...Continue Reading
Citations
Feb 5, 2016·Journal of Experimental Botany·Belén NaranjoFrancisco Javier Cejudo
Oct 24, 2015·Protein Expression and Purification·Yuki OkegawaKen Motohashi
Jun 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Keisuke Yoshida, Toru Hisabori
Feb 3, 2016·Plant, Cell & Environment·Lauri NikkanenEevi Rintamäki
Aug 4, 2016·Biochimica Et Biophysica Acta·Cara A GriffithsChristine H Foyer
May 29, 2016·The Plant Journal : for Cell and Molecular Biology·L Ruby CarrilloDavid M Kramer
Sep 13, 2016·International Journal of Molecular Sciences·Fuwang WuXuewu Duan
Dec 13, 2016·Molecular Plant·Ina ThormählenPeter Geigenberger
Jul 12, 2017·Plant Signaling & Behavior·Valle OjedaFrancisco J Cejudo
Jul 15, 2017·Plant, Cell & Environment·Vanesa B TognettiMónika Hrtyan
Jun 20, 2017·Plant, Cell & Environment·Virginie MenginMark Stitt
Oct 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Juan Manuel Pérez-RuizFrancisco Javier Cejudo
Mar 20, 2019·Plant Signaling & Behavior·Paula Da Fonseca-PereiraWagner L Araújo
Jan 18, 2020·PLoS Computational Biology·Melanie GerkenKarl-Josef Dietz
Aug 6, 2019·Plant, Cell & Environment·Paula da Fonseca-PereiraDanilo M Daloso
Aug 16, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lauri NikkanenEevi Rintamäki
Jul 12, 2019·Journal of Experimental Botany·Maricruz GonzálezFrancisco Javier Cejudo
Aug 5, 2018·FEBS Letters·Andreas S RichterBernhard Grimm
Aug 11, 2020·The Plant Journal : for Cell and Molecular Biology·Manuel Guinea DiazEevi Rintamäki
Apr 26, 2019·Frontiers in Plant Science·Francisco Javier CejudoJuan Manuel Pérez-Ruiz
Nov 30, 2019·Plants·María AncínInmaculada Farran
Apr 17, 2019·The Biochemical Journal·Lauri Nikkanen, Eevi Rintamäki
Dec 20, 2018·International Journal of Molecular Sciences·Xi ChenJingkui Tian
Nov 6, 2018·Antioxidants·Daniel WittmannBernhard Grimm
Sep 4, 2020·International Journal of Molecular Sciences·Hubert SytykiewiczKatarzyna Kmieć
Oct 18, 2020·Biological Chemistry·Daniel WittmannBernhard Grimm
Oct 23, 2020·Plants·Mohammed ElasadHengling Wei
Nov 5, 2020·Antioxidants·Ana Jurado-FloresFrancisco Javier Cejudo
Jun 5, 2019·Bio-protocol·Keisuke Yoshida, Toru Hisabori
Jul 3, 2021·Antioxidants·Belen NaranjoDario Leister
Aug 24, 2021·Frontiers in Plant Science·M Luisa Hernández, Francisco Javier Cejudo
Nov 10, 2021·Journal of Natural Products·Ricardo ColonKathleen S Rein
Jun 5, 2019··Toru HisaboriKeisuke Yoshida