PMID: 9546607Apr 18, 1998Paper

Third calcium-modulated rod outer segment membrane guanylate cyclase transduction mechanism

Molecular and Cellular Biochemistry
A KrishnanRameshwar K Sharma

Abstract

Ca2+-modulated rod outer segment membrane guanylate cyclase (ROS-GC1) has been cloned and reconstituted to show that it is regulated by two processes: one inhibitory, the other stimulatory. The inhibitory process is consistent with its linkage to phototransduction; the physiology of the stimulatory process is probably linked to neuronal transmission. In both regulatory processes, calcium modulation of the cyclase takes place through the calcium binding proteins; guanylate cyclase activating proteins (GCAP1 and GCAP2) in the case of the phototransduction process and calcium-dependent GCAP (CD-GCAP) in the case of the stimulatory process. The cyclase domains involved in the two processes are located at two different sites on the ROS-GC1 intracellular region. The GCAP1-modulated domain resides within the aa 447-730 segment of ROS-GC1 and the CD-GCAP-modulated domain resides within the aa 731-1054 segment. In the present study the GCAP2-dependent Ca2+ modulation of the cyclase activity has been reconstituted using recombinant forms of GCAP2 and ROS-GC1, and its mutants. The results indicate that consistent to phototransduction, GCAP2 at low Ca2+ concentration (10 nM) maximally stimulates the cyclase activity of the wild-type and it...Continue Reading

Citations

Dec 3, 2009·Molecular and Cellular Biochemistry·Rameshwar K Sharma, Teresa Duda
Jul 30, 2014·Frontiers in Molecular Neuroscience·Rameshwar K Sharma, Teresa Duda
Feb 10, 2016·Frontiers in Molecular Neuroscience·Teresa DudaRameshwar K Sharma
Aug 28, 1999·The Journal of Biological Chemistry·E V OlshevskayaA M Dizhoor
Nov 17, 2009·Molecular and Cellular Biochemistry·Anna Jankowska, Jerzy B Warchol
Oct 4, 2016·Frontiers in Molecular Neuroscience·Rameshwar K SharmaClint L Makino
Dec 4, 2009·Molecular and Cellular Biochemistry·Rameshwar K Sharma
Feb 11, 2018·Molecular and Cellular Biochemistry·Teresa DudaRameshwar K Sharma
Apr 12, 2014·Frontiers in Molecular Neuroscience·Rameshwar K SharmaTeresa Duda
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·C L TuckerJ B Hurley
May 8, 1998·Biochemical and Biophysical Research Communications·R M GoraczniakR K Sharma
Dec 3, 1999·Methods : a Companion to Methods in Enzymology·A M Dizhoor, J B Hurley
May 27, 2008·Biochemistry·Venkateswar VenkataramanRameshwar K Sharma

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