Third-generation electrokinetically pumped sheath-flow nanospray interface with improved stability and sensitivity for automated capillary zone electrophoresis-mass spectrometry analysis of complex proteome digests

Journal of Proteome Research
Liangliang SunNorman J Dovichi

Abstract

We have reported a set of electrokinetically pumped sheath flow nanoelectrospray interfaces to couple capillary zone electrophoresis with mass spectrometry. A separation capillary is threaded through a cross into a glass emitter. A side arm provides fluidic contact with a sheath buffer reservoir that is connected to a power supply. The potential applied to the sheath buffer drives electro-osmosis in the emitter to pump the sheath fluid at nanoliter per minute rates. Our first-generation interface placed a flat-tipped capillary in the emitter. Sensitivity was inversely related to orifice size and to the distance from the capillary tip to the emitter orifice. A second-generation interface used a capillary with an etched tip that allowed the capillary exit to approach within a few hundred micrometers of the emitter orifice, resulting in a significant increase in sensitivity. In both the first- and second-generation interfaces, the emitter diameter was typically 8 μm; these narrow orifices were susceptible to plugging and tended to have limited lifetime. We now report a third-generation interface that employs a larger diameter emitter orifice with very short distance between the capillary tip and the emitter orifice. This modified ...Continue Reading

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Citations

Oct 27, 2015·Journal of Separation Science·Sille Štěpánová, Václav Kašička
Jan 12, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mei HanDan A Rock
Oct 29, 2015·Proteomics·Liangliang SunNorman J Dovichi
Dec 15, 2015·Bioanalysis·Roza WojcikNorman J Dovichi
Feb 6, 2016·Electrophoresis·Víctor González-RuizJulie Schappler
Apr 29, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nicole M SchiavoneNorman J Dovichi
Jul 28, 2016·Current Opinion in Biotechnology·Wei ZhangRawi Ramautar
Oct 27, 2016·Electrophoresis·Anna TýčováKarel Klepárník
Nov 1, 2016·Electrophoresis·Rawi RamautarGerhardus J de Jong
Nov 18, 2016·Journal of the American Society for Mass Spectrometry·Sam B ChoiPeter Nemes
Dec 10, 2016·Journal of Separation Science·Guijie ZhuNorman J Dovichi
May 5, 2017·The Analyst·Mohamed DawodRobert T Kennedy
Mar 2, 2018·Electrophoresis·Akiyoshi HirayamaTomoyoshi Soga
Apr 6, 2018·Cancer & Metabolism·Chen DaiLaurie E Littlepage
Nov 11, 2017·Electrophoresis·Víctor González-RuizJulie Schappler
Jul 20, 2018·Rapid Communications in Mass Spectrometry : RCM·Rabah GahoualYannis-Nicolas François
Aug 14, 2019·Mass Spectrometry Reviews·Fabio P Gomes, John R Yates
May 3, 2019·Proteomics·Leah V SchafferLloyd M Smith
Jan 21, 2020·Journal of Mass Spectrometry : JMS·Alan A Doucette, Jessica L Nickerson
May 20, 2020·Expert Review of Proteomics·Alain Van GoolMarei Sammar
Oct 17, 2019·Glycobiology·Xiaorui HanRobert J Linhardt
Aug 13, 2017·Electrophoresis·Yuanyuan LiuChao Yan

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