PMID: 2495439Apr 1, 1989Paper

Thirty compounds tested in the Drosophila wing spot test

Mutation Research
Ulrich GrafF E Würgler

Abstract

The Drosophila wing somatic mutation and recombination test (SMART) was evaluated for its suitability in genotoxicity screening by testing 30 chemicals. Of the 2 crosses used, the mwh-flr3 cross turned out to be more convenient than the previously used mwh-flr cross. Based on the experience gained with both acute exposures and chronic exposures of different duration, we suggest that the optimal strategy in genotoxicity screening is to start with chronic exposure of 3-day-old larvae for 48 h (that is, until pupation). Only for unstable compounds and very volatile compounds and gases are acute treatments, including inhalation, recommended. In general, a qualitative evaluation of the genotoxicity of a compound in the wing assay is possible with as few as 1-2 different exposure concentrations. A more quantitative evaluation of genotoxicity, based upon dose-response data, can often be achieved with as few as 3-4 concentrations. The results reported here were obtained in 2 different laboratories, demonstrating that the wing spot test is easily transferable to other laboratories. The experience gained indicates that the assay has now been developed to an extent that a coordinated international comparative validation study is desirable.

References

Sep 1, 1976·Mutation Research·D V Martensen, M M Green
Jan 1, 1974·Molecular & General Genetics : MGG·A Garcia-Bellido, J Dapena
Jun 1, 1983·Mutation Research·U GrafF E Würgler
Jan 1, 1984·Environmental Mutagenesis·U GrafP G Kale

❮ Previous
Next ❯

Citations

Aug 5, 2011·American Journal of Medical Genetics. Part a·Marco CastoriPaola Grammatico
Dec 1, 1992·Mutation Research·E W Vogel
Oct 1, 1995·Mutation Research·J A FerreiroM A Comendador
Oct 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J SchlatterU Graf
May 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·U GrafE Díaz Carrillo
Jan 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S K Abraham, U Graf
Sep 5, 1998·Mutation Research·U GrafF E Würgler
Dec 12, 1996·Mutation Research·M HersbergerR M Widmer
Aug 6, 2002·Mutation Research·Marcelo TiburiHeloísa Helena Rodrigues de Andrade
May 11, 2011·Journal of Medicinal Food·América Castañeda SortibránRosario Rodríguez-Arnaiz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.