Thixotropic Peptide-Based Physical Hydrogels Applied to Three-Dimensional Cell Culture

ACS Omega
Nicola ZannaClaudia Tomasini

Abstract

Pseudopeptides containing the d-Oxd or the d-pGlu [Oxd = (4R,5S)-4-methyl-5-carboxyl-oxazolidin-2-one, pGlu = pyroglutamic acid] moiety and selected amino acids were used as low-molecular-weight gelators to prepare strong and thixotropic hydrogels at physiological pH. The addition of calcium chloride to the gelator solutions induces the formation of insoluble salts that get organized in fibers at a pH close to the physiological one. Physical characterization of hydrogels was carried out by morphologic evaluation and rheological measurements and demonstrated that the analyzed hydrogels are thixotropic, as they have the capability to recover their gel-like behavior. As these hydrogels are easily injectable and may be used for regenerative medicine, they were biologically assessed by cell seeding and viability tests. Human gingival fibroblasts were embedded in 2% hydrogels; all of the hydrogels allow the growth of encapsulated cells with a very good viability. The gelator toxicity may be correlated with their tendency to self-assemble and is totally absent when the hydrogel is formed.

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Citations

Oct 24, 2017·Biopolymers·Gaetano AngeliciClaudia Tomasini
Jan 28, 2020·Soft Matter·Sanjoy MondalArun K Nandi
Oct 21, 2017·Gels·Nicola Zanna, Claudia Tomasini
Dec 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Demetra GiuriClaudia Tomasini
Apr 18, 2018·Chemistry : a European Journal·Henrik SundénAnna Stöm
Jul 1, 2017·Organic & Biomolecular Chemistry·Nicola ZannaClaudia Tomasini
Oct 31, 2017·Soft Matter·Michael C NolanDave J Adams
Jan 9, 2018·Royal Society Open Science·Yutaka OhsedoNobuyoshi Miyamoto
May 1, 2021·International Journal of Molecular Sciences·Yusuke SugiokaAyae Sugawara-Narutaki
May 1, 2021·Gels·Paolo RavarinoClaudia Tomasini
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fanny d'OrlyéAnne Varenne
Aug 29, 2019·ACS Omega·Gloria GuidettiClaudia Tomasini
Oct 29, 2020·ACS Omega·Vivek PrakashVibin Ramakrishnan

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Methods Mentioned

BETA
scanning electron microscopy
FCS

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