Thr(207) of claudin-5 is involved in size-selective loosening of the endothelial barrier by cyclic AMP

Experimental Cell Research
Tamotsu SomaNorimasa Sawada

Abstract

We have recently shown that cyclic AMP (cAMP) increases claudin-5 immunoreactivity along cell boundaries and could promote phosphorylation of claudin-5 on threonine residues in porcine blood-brain barrier (BBB) endothelial cells via a protein kinase A (PKA)-dependent pathway (Exp. Cell Res. 290 [2003] 275). Along this line, we identified a putative phosphorylation site for PKA at Thr(207) in the intracytoplasmic carboxyl terminal domain of claudin-5. To clarify the biological significance of this site in regulation of endothelial barrier functions, we established rat lung endothelial (RLE) cells expressing doxycycline (Dox)-inducible wild-type claudin-5 and a mutant with a substitution of Ala for Thr(207) (CL5T207A). We show that induction of wild-type claudin-5 is sufficient to reconstitute the paracellular barrier against inulin (5 kDa), but not mannitol (182 Da), in leaky RLE cells. By contrast, the barrier against both molecules was induced in the mutant cells. We also demonstrate that, upon cAMP treatment, Thr(207) of claudin-5 is involved in enhancement of claudin-5 immunoreactive signals along cell borders, rapid reduction in transendothelial electrical resistance (TER), and loosening of the claudin-5-based endothelial b...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Dec 1, 1991·The Journal of Cell Biology·L L RubinJ Morales
Apr 1, 1989·Journal of Cellular Physiology·T J StelznerR F O'Brien
Oct 1, 1988·Molecular and Cellular Biochemistry·B R StevensonS Bullivant
Dec 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D A Walsh, S M Van Patten
Dec 1, 1993·The Journal of Cell Biology·M FuruseS Tsukita
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·K MoritaS Tsukita
Apr 15, 1999·Annual Review of Neuroscience·L L Rubin, J M Staddon
Oct 29, 2000·The Journal of Biological Chemistry·M Aurrand-LionsB A Imhof
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·S TsukitaM Itoh
Jan 26, 2002·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·Shin-ichi Atsumi SiMichio Mori
Feb 16, 2002·The Journal of Biological Chemistry·Ines NasdalaStefan Butz
Mar 26, 2002·Journal of the American Society of Nephrology : JASN·Yumiko Kiuchi-SaishinShoichiro Tsukita
Jan 18, 2003·Vascular Pharmacology·Hartwig Wolburg, Andrea Lippoldt
May 14, 2003·The Journal of Cell Biology·Takehiro NittaShoichiro Tsukita
Aug 12, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carolyn B CoyneLarry G Johnson
Dec 6, 2003·Journal of Cell Science·Klaus EbnetDietmar Vestweber
May 26, 2004·Journal of Cell Science·Kursad Turksen, Tammy-Claire Troy

❮ Previous
Next ❯

Citations

Oct 21, 2006·The Journal of Clinical Investigation·Tatiana KisselevaChristian Schindler
Aug 30, 2008·World Journal of Gastroenterology : WJG·Yong-Lian WuYu-Peng Chen
Aug 14, 2012·International Journal of Legal Medicine·Qi WangHitoshi Maeda
Jun 8, 2010·American Journal of Respiratory Cell and Molecular Biology·An Soo JangGeorge D Leikauf
Jul 12, 2012·Cellular and Molecular Life Sciences : CMLS·Takuya Suzuki
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Yuri PersidskyGeorgette D Kanmogne
Nov 13, 2012·Fluids and Barriers of the CNS·Anny-Claude LuissintPierre-Olivier Couraud
Dec 19, 2015·Journal of Proteome Research·Fei LiuMark S Baker
May 21, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Keith D RochfortPhilip M Cummins
Apr 17, 2012·Journal of Drug Delivery·Koji HanaiNorimasa Sawada
Mar 21, 2006·Journal of Cellular Physiology·Kunihiko NagasawaNorimasa Sawada
Sep 9, 2006·European Journal of Cell Biology·Ruud D FontijnAnton J G Horrevoets
May 4, 2016·Tissue Barriers·Svetlana M StamatovicAnuska V Andjelkovic
Oct 6, 2007·Biochimica Et Biophysica Acta·Hideki ChibaNorimasa Sawada
Mar 26, 2014·Tissue Barriers·Andreia GonçalvesRosa Fernandes
Nov 3, 2007·Biochimica Et Biophysica Acta·Akira IkariMasao Miwa
Dec 17, 2014·Journal of Molecular Cell Biology·Ruo-Lan XiangGuang-Yan Yu
Feb 21, 2006·International Review of Cytology·Saima AijazKarl Matter
May 24, 2005·Mechanisms of Development·Tammy-Claire TroyKursad Turksen
Oct 24, 2007·Biochimica Et Biophysica Acta·Lorenza González-MariscalDavid Chamorro
Aug 25, 2017·Tissue Barriers·Amanda I BaumholtzAimee K Ryan
May 24, 2014·Chonnam Medical Journal·An-Soo Jang
Nov 20, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xing-He WengAndrea Quaroni
Sep 9, 2006·The Journal of Physiological Sciences : JPS·Akira IkariMasao Miwa
Feb 4, 2010·Cellular and Molecular Neurobiology·Yoichi MorofujiMasami Niwa
Jul 24, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Salim Abdelilah-Seyfried
Dec 12, 2012·Cellular and Molecular Neurobiology·Shoji HoraiMasami Niwa
Apr 17, 2013·Physiological Reviews·Dorothee Günzel, Alan S L Yu
Feb 25, 2020·Current Pharmaceutical Design·Yoichi Morofuji, Shinsuke Nakagawa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.