Three active forms of aspartic proteinase from Mason-Pfizer monkey virus

Virology
A ZábranskýIva Pichová

Abstract

Mason-Pfizer monkey virus (M-PMV) proteinase, released by the autocatalytic cleavage of Gag-Pro and Gag-Pro-Pol polypeptide precursors, catalyzes the processing of viral precursors to yield the structural proteins and enzymes of the virion. In retroviruses, usually only one proteolytically active form of proteinase exists. Here, we describe an unusual feature of M-PMV, the existence of three active forms of a retroviral proteinase with molecular masses of 17, 13, and 12 kDa as determined by mass spectroscopy. These forms arise in vitro by self-processing of a 26-kDa proteinase precursor. We have developed a process for isolation of each truncated product and demonstrate that all three forms display proteolytic activity. Amino acid analyses, as well as the determination of N- and C-terminal sequences, revealed that the N-termini of all three forms are identical, confirming that in vitro autoprocessing of the 17-kDa form occurs at the C-terminus to yield the truncated forms. The 17-kDa form and the newly described 13-kDa form of proteinase were identified in virions collected from the rhesus monkey CMMT cell line chronically infected with M-PMV, confirming that multiple forms exist in vivo.

References

Mar 1, 1992·Journal of Virology·J H ElderT R Phillips
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A H Kaplan, R Swanstrom
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R L ShoemanM C Graves
Jun 23, 1995·The Journal of Biological Chemistry·O Hrusková-HeidingsfeldováE Hunter
Jan 1, 1993·Annual Review of Biochemistry·A Wlodawer, J W Erickson

❮ Previous
Next ❯

Citations

Nov 22, 2011·Acta Crystallographica. Section D, Biological Crystallography·Miroslaw GilskiMariusz Jaskolski
Dec 16, 2011·Journal of Virology·Ivana KřízováIva Pichová
Jun 14, 2000·Archives of Biochemistry and Biophysics·J SnáselI Pichová
Mar 26, 2014·Protein Expression and Purification·Tomáš KroupaRichard Hrabal
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·S D Parker, E Hunter
Jan 7, 2005·The FEBS Journal·Jan SnáselIva Pichová
Oct 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S NafaF Zoulim
Oct 22, 2003·Journal of Molecular Biology·Václav VeverkaRichard Hrabal
May 29, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C T YehY F Liaw
Dec 5, 2006·Journal of Molecular Biology·Helena ZábranskáIva Pichová
Sep 23, 2003·Protein Science : a Publication of the Protein Society·Marek IngrJan Konvalinka
Aug 30, 2005·Analytical Biochemistry·Dana HorákováTomás Ruml
Mar 25, 2014·The Journal of General Virology·Michaela RumlováTomáš Ruml
Oct 8, 2019·Acta Crystallographica. Section D, Structural Biology·Stanislaw WosickiMariusz Jaskolski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.