Three alpha-subunits of heterotrimeric G proteins and an adenylyl cyclase have distinct roles in fruiting body development in the homothallic fungus Sordaria macrospora.

Genetics
Jens KamerewerdUlrich Kück

Abstract

Sordaria macrospora, a self-fertile filamentous ascomycete, carries genes encoding three different alpha-subunits of heterotrimeric G proteins (gsa, G protein Sordaria alpha subunit). We generated knockout strains for all three gsa genes (Deltagsa1, Deltagsa2, and Deltagsa3) as well as all combinations of double mutants. Phenotypic analysis of single and double mutants showed that the genes for Galpha-subunits have distinct roles in the sexual life cycle. While single mutants show some reduction of fertility, double mutants Deltagsa1Deltagsa2 and Deltagsa1Deltagsa3 are completely sterile. To test whether the pheromone receptors PRE1 and PRE2 mediate signaling via distinct Galpha-subunits, two recently generated Deltapre strains were crossed with all Deltagsa strains. Analyses of the corresponding double mutants revealed that compared to GSA2, GSA1 is a more predominant regulator of a signal transduction cascade downstream of the pheromone receptors and that GSA3 is involved in another signaling pathway that also contributes to fruiting body development and fertility. We further isolated the gene encoding adenylyl cyclase (AC) (sac1) for construction of a knockout strain. Analyses of the three DeltagsaDeltasac1 double mutants an...Continue Reading

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Citations

Jan 31, 2012·Applied and Environmental Microbiology·André SchusterMonika Schmoll
Aug 27, 2010·European Journal of Cell Biology·Ines EnghUlrich Kück
Jun 11, 2011·FEMS Microbiology Reviews·Ozgür Bayram, Gerhard H Braus
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Mar 13, 2020·Applied Microbiology and Biotechnology·Ines TeichertMinou Nowrousian
Apr 16, 2015·G3 : Genes - Genomes - Genetics·Stefanie Traeger, Minou Nowrousian
Sep 30, 2021·Microbiology and Molecular Biology Reviews : MMBR·Andi M WilsonBrenda D Wingfield

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