PMID: 8445724Apr 1, 1993Paper

Three baculovirus genes involved in late and very late gene expression: ie-1, ie-n, and lef-2

Journal of Virology
A L Passarelli, L K Miller

Abstract

We developed a method to identify baculovirus genes required for late and very late gene expression that is based on subtraction of clones from an Autographa californica nuclear polyhedrosis virus genomic library which is able to trans activate promoters of reporter plasmids in transient expression assays. Using this assay, we found that three genes located between 83.7 and 7.5 map units of the Autographa californica nuclear polyhedrosis virus genome were involved in expression from the late capsid protein (vp39) and very late polyhedrin (polh) gene promoters. Two of these genes, ie-1 and ie-n, trans regulate early genes in transient expression assays. Although ie-1 was necessary and sufficient for expression from the early promoter in our assay, it was necessary but not sufficient for expression from the vp39 and polh promoters. The presence of ie-n increased expression from the early, late, and very late classes of promoters tested. However, a third gene identified in this region was specifically required for expression from the vp39 and polh promoters. This gene, a previously sequenced 630-nucleotide open reading frame, was renamed lef-2 for late expression factor 2. We also found that other genes in the region between 83.7 ...Continue Reading

References

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Citations

Jun 18, 2003·The Journal of General Virology·Jane S TitteringtonA Lorena Passarelli
Aug 21, 2002·Journal of Virology·Erin A Crouch, A Lorena Passarelli
Dec 14, 2001·The Journal of Biological Chemistry·Huei-Ru LoYu-Chan Chao
Aug 9, 2007·Virus Genes·Marcos Fabián BilenPablo Daniel Ghiringhelli
Oct 1, 2013·Molecular Biology Reports·Yejun WuWenbing Wang
Jan 1, 1997·Annual Review of Genetics·J Flint, T Shenk
Jul 21, 2004·Journal of Molecular Microbiology and Biotechnology·Kathleen L Hefferon
Dec 28, 2002·Journal of Virology·Noriko ImaiWonKyung Kang
Nov 11, 2006·Virus Genes·Qianyun XiXunzhang Wang
Nov 29, 2015·Genome Biology and Evolution·Anderson Fernandes de BritoPaolo Marinho de Andrade Zanotto
Sep 22, 2018·Viruses·Chikako OnoYoshiharu Matsuura
Jan 7, 1998·Journal of Virology·E A Prikhod'ko, L K Miller
Jul 10, 1999·Journal of Virology·E A Prikhod'ko, L K Miller
Feb 11, 1999·Journal of Virology·E A Prikhod'koL K Miller
Sep 22, 2000·Virology·Y HashimotoT Matsumoto
Sep 30, 1999·Virology·T HayakawaS Maeda
Jul 1, 1997·Journal of Invertebrate Pathology·M M Van Oers, J M Vlak

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