PMID: 7889949Oct 1, 1994

Three beta-lactamases isolated from Aeromonas salmonicida, including a carbapenemase not detectable by conventional methods

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
M V HayesS G Amyes

Abstract

The beta-lactamases of seven strains of Aeromonas salmonicida subsp. achromogenes resistant to amoxicillin (MIC > 1024 mg/l) and responsible for furunculosis in farmed Atlantic salmon in Scotland were examined to establish the mechanisms of beta-lactam resistance. Separation of a cell-free extract on an isoelectric focusing gel stained with the chromogenic cephalosporin nitrocefin showed the presence of two beta-lactamases, one with a pI of 7.9 and the other with a pI of 6.0. Hydrolysis assays of cell-free extracts of these strains demonstrated carbapenemase, penicillinase and cephalosporinase activity. However, when the beta-lactamases were separated by anion exchange chromatography, the carbapenemase activity could not be retrieved in either of the peak fractions containing the separated enzymes that had been visualised by nitrocefin. Consequently, a novel carbapenemase was discovered which cannot be detected with nitrocefin.

References

Apr 6, 2002·FEMS Immunology and Medical Microbiology·Lara SolerJosep Guarro
Jul 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·G M RossoliniG Amicosante
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·George A Jacoby
Jul 17, 2007·Clinical Microbiology Reviews·Anne Marie Queenan, K Bush
Jan 13, 2009·Clinical Microbiology Reviews·George A Jacoby
Sep 22, 2006·Acta Veterinaria Scandinavica·Seppo A M Saari, Sven E Nikander
Oct 11, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·S KirkanO Kaya
May 22, 2018·BioMed Research International·Sadeeq Ur RahmanJian Gao

Citations

Sep 1, 1992·Antimicrobial Agents and Chemotherapy·D M Livermore, J E Corkill
Dec 1, 1991·Antimicrobial Agents and Chemotherapy·A C BarnesS G Amyes
Jan 1, 1990·Antimicrobial Agents and Chemotherapy·D A Weber, C C Sanders
Jan 1, 1990·Antimicrobial Agents and Chemotherapy·J P Iaconis, C C Sanders
Jun 1, 1989·Antimicrobial Agents and Chemotherapy·L GutmannE Collatz
Jul 1, 1988·Reviews of Infectious Diseases·L GutmannR Williamson
Aug 1, 1986·Antimicrobial Agents and Chemotherapy·A J Reid, S G Amyes
Aug 1, 1985·Antimicrobial Agents and Chemotherapy·C KliebeB Wiedemann
May 16, 1980·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R P Ambler
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B Jaurin, T Grundström
Jul 1, 1950·Journal of Bacteriology·B D DAVIS, E S MINGIOLI

Related Concepts

Salmo salar
Carbapenemase
Bacterial Proteins
beta-Lactams
Amoxicillin
CfiA protein, Bacteroides fragilis
Enzymes, antithrombotic
SDS-PAGE
Chromatography
Wymox

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