Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σ(A)) and alternative SigI6 (σ(I6)) factors

FEBS Letters
Andy SandY Shoham

Abstract

Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the σ(I6)/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative σ(I6) and σ(A) vegetative promoters.

References

Jan 1, 1991·Journal of Bacteriology·S MishraJ P Aubert
Feb 1, 1986·Molecular & General Genetics : MGG·P BéguinJ P Aubert
Aug 1, 1985·Journal of Bacteriology·E A BayerR Lamed
Nov 1, 1983·Journal of Bacteriology·E A BayerR Lamed
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·K KruusJ H Wu
Sep 1, 1994·Trends in Biotechnology·E A BayerR Lamed
Mar 1, 1997·Applied and Environmental Microbiology·L GalJ P Belaich
Aug 19, 2003·Journal of Bacteriology·Tali W DrorYuval Shoham
Oct 19, 2004·Annual Review of Microbiology·Edward A BayerRaphael Lamed
Dec 17, 2004·Journal of Bacteriology·Yi-Heng Percival Zhang, Lee R Lynd
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Arnold L DemainJ H David Wu
Dec 23, 2006·Microbiology·Kei AsaiYoshito Sadaie
Feb 10, 2007·Science·José Goldemberg
May 15, 2008·Applied Biochemistry and Biotechnology·Steven D BrownJonathan R Mielenz
Jun 6, 2008·Current Opinion in Biotechnology·Sara E Blumer-SchuetteRobert M Kelly
Oct 28, 2008·Journal of Bacteriology·Yakir NatafYuval Shoham
Dec 25, 2008·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Edward A BayerHarry J Flint
Apr 9, 2010·Annual Review of Biochemistry·Carlos M G A Fontes, Harry J Gilbert
Sep 8, 2010·Journal of Industrial Microbiology & Biotechnology·Liat BahariEdward A Bayer
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yakir NatafYuval Shoham
Nov 26, 2010·The Journal of Biological Chemistry·Yannick J BombleMichael F Crowley
Dec 25, 2010·Applied Microbiology and Biotechnology·Anuj K Chandel, Om V Singh
Feb 15, 2011·Applied Microbiology and Biotechnology·Michael NewcombJ H David Wu
Jun 16, 2011·BMC Microbiology·Babu RamanJonathan R Mielenz
Oct 4, 2011·Applied and Environmental Microbiology·D Aaron ArgyrosNicky C Caiazza
Feb 22, 2012·Journal of Industrial Microbiology & Biotechnology·Evert K HolwerdaLee R Lynd
May 23, 2012·Methods in Enzymology·Daniel G Olson, Lee R Lynd
Jun 8, 2012·Standards in Genomic Sciences·Javier A IzquierdoLee R Lynd
Jun 19, 2013·Current Opinion in Structural Biology·Harry J GilbertAlisdair B Boraston
Jul 9, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Oren YanivLinda J W Shimon
Aug 8, 2013·Journal of Molecular Microbiology and Biotechnology·Jungu BaeMitsuyoshi Ueda
Oct 2, 2013·Current Opinion in Structural Biology·Steven P Smith, Edward A Bayer

❮ Previous
Next ❯

Citations

Feb 6, 2017·Applied and Environmental Microbiology·Lizett Ortiz de OraIlya Borovok
Jun 5, 2020·The Journal of Biological Chemistry·Smadar ShulamiYuval Shoham
Aug 24, 2017·Bioscience, Biotechnology, and Biochemistry·Shunsuke IchikawaShuichi Karita
Jul 25, 2018·Scientific Reports·Lizett Ortiz de OraIván Muñoz-Gutiérrez
Dec 15, 2020·International Journal of Biological Macromolecules·Nisha SinghMunish Puri

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.