Three-component Langmuir-Blodgett films consisting of surfactant, clay mineral, and lysozyme: construction and characterization

Chemistry : a European Journal
Shiding MiaoRobert Schoonheydt

Abstract

The Langmuir-Blodgett (L-B) technique has been employed for the construction of hybrid films consisting of three components: surfactant, clay, and lysozyme (Lys). The surfactants are octadecylammonium chloride (ODAH) and octadecyl ester of rhodamine B (RhB18). The clays include saponite and laponite. Surface pressure versus area isotherms indicate that lysozyme is adsorbed by the surfactant-clay L-B film at the air-water interface without phase transition. The UV-visible spectra of the hybrid film ODAH-saponite-Lys show that the amount of immobilized lysozyme in the hybrid film is (1.3+/-0.2) ng mm(-2). The average surface area (Omega) per molecule of lysozyme is approximately 18.2 nm(2) in the saponite layer. For the multilayer film (ODAH-saponite-Lys)(n), the average amount of lysozyme per layer is (1.0+/-0.1) ng mm(-2). The amount of lysozyme found in the hybrid films of ODAH-laponite-Lys is at the detection limit of about 0.4 ng mm(-2). Attenuated total reflectance (ATR) FTIR spectra give evidence for clay layers, ODAH, lysozyme, and water in the hybrid film. The octadecylammonium cations are partially oxidized to the corresponding carbamate. A weak 1620 cm(-1) band of lysozyme in the hybrid films is reminiscent of the pres...Continue Reading

References

May 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·I M Pepe, C Nicolini
Aug 18, 2001·Science·D Y TakamotoP S Cremer
Feb 19, 2002·Chemical Reviews·JoAnne Stubbe, Wilfred A. van Der Donk
Dec 12, 2002·Chemical Reviews·Philip N Bryan
Jan 30, 2004·Biophysical Journal·Marcel BenzJacob Israelachvili
Jun 24, 2004·Journal of the American Chemical Society·Nurettin DemirdövenAndrei Tokmakoff
Apr 20, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Luciano CaseliM Elisabete D Zaniquelli
Aug 11, 2005·Chemical Communications : Chem Comm·Robin H A RasRobert A Schoonheydt
Sep 27, 2005·Advances in Colloid and Interface Science·Agnès P Girard-EgrotLoïc J Blum
Jan 24, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·E PechkovaC Nicolini
Feb 3, 2007·The Journal of Physical Chemistry. B·Tapanendu KamilyaG B Talapatra
Feb 16, 2007·Physical Chemistry Chemical Physics : PCCP·Robin H A RasRobert A Schoonheydt
Mar 31, 2007·Journal of the American Chemical Society·Xiaolin LiHongjie Dai
Apr 20, 2007·Accounts of Chemical Research·Xiaodong ChenLifeng Chi
Aug 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Søren Prip BeierGunnar Jonsson
Feb 2, 2008·The Journal of Physical Chemistry. B·Kazuhiko KandoriShintaro Tsuyama
Feb 22, 2008·Accounts of Chemical Research·Ziad GanimAndrei Tokmakoff
Sep 5, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Eduardo M ClopMaría A Perillo
Sep 5, 2008·Journal of the American Chemical Society·Mikko J ParryPaavo K J Kinnunen
May 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Luciano CaseliOsvaldo N Oliveira

❮ Previous
Next ❯

Citations

Oct 6, 2015·The Journal of Physical Chemistry. B·Mei HuangShiding Miao
Oct 1, 2014·The Journal of Physical Chemistry. B·Shulian HeShiding Miao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.