Three different and tissue-specific NAD-malic enzymes generated by alternative subunit association in Arabidopsis thaliana.

The Journal of Biological Chemistry
Marcos A TronconiMaría F Drincovich

Abstract

The Arabidopsis thaliana genome contains two genes encoding the mitochondrial NAD-malic enzyme (NAD-ME), NAD-ME1 (At2g13560) and NAD-ME2 (At4g00570). The characterization of recombinant NAD-ME1 and -2 indicated that both enzymes assemble as active homodimers; however, a heterodimeric enzyme (NAD-MEH) can also be detected by electrophoretic studies. To analyze the metabolic contribution of each enzymatic entity, NAD-MEH was obtained by a co-expression-based recombinant approach, and its kinetic and regulatory properties were analyzed. The three NAD-MEs show similar kinetic properties, although they differ in the regulation by several metabolic effectors. In this regard, whereas fumarate activates NAD-ME1 and CoA activates NAD-ME2, both compounds act synergistically on NAD-MEH activity. The characterization of two chimeric enzymes between NAD-ME1 and -2 allowed specific domains of the primary structure, which are involved in the differential allosteric regulation, to be identified. NAD-ME1 and -2 subunits showed a distinct pattern of accumulation in the separate components of the floral organ. In sepals, the NAD-ME1 subunit is present at a slightly higher proportion than the NAD-ME2 subunit, and thus, NAD-MEH and NAD-ME1 act in c...Continue Reading

References

May 1, 1977·Analytical Biochemistry·J J Sedmak, S E Grossberg
Mar 1, 1984·Archives of Biochemistry and Biophysics·P F Canellas, R T Wedding
Sep 18, 2004·Plant Physiology·Philip ZimmermannWilhelm Gruissem
Nov 24, 2004·Plant Physiology·Fatma KaplanCharles L Guy
Dec 18, 2004·Plant Molecular Biology·Mariana SaigoMaría F Drincovich
Aug 23, 2005·Plant Physiology·Mariel C Gerrard WheelerVerónica G Maurino
Apr 10, 2007·Biochimica Et Biophysica Acta·Lidia de BariSalvatore Passarella
Feb 15, 2008·Plant & Cell Physiology·Gabriela Leticia MüllerMaría Valeria Lara

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Citations

Apr 5, 2011·Journal of Experimental Botany·Alexandra MaierVeronica G Maurino
Sep 19, 2015·The Arabidopsis Book·Veronica G Maurino, Martin K M Engqvist
May 9, 2014·Frontiers in Plant Science·Peter Schertl, Hans-Peter Braun
Jun 26, 2013·International Journal of Molecular Sciences·Jinwen LiuYuxiang Cheng
Sep 15, 2019·The Plant Journal : for Cell and Molecular Biology·Philippe FuchsMarkus Schwarzländer
Jun 28, 2016·PloS One·Mariel Claudia Gerrard WheelerMariana Saigo
Sep 5, 2020·Isotopes in Environmental and Health Studies·Shiva GhiasiRoland A Werner
Apr 14, 2018·Acta Crystallographica. Section D, Structural Biology·C E AlvarezA Buschiazzo
Apr 12, 2020·Mitochondrion·Abir U Igamberdiev
May 10, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Nils RugenHolger Eubel

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