Three dimensional accurate morphology measurements of polystyrene standard particles on silicon substrate by electron tomography

Micron : the International Research and Review Journal for Microscopy
Misa HayashidaMarek Malac

Abstract

Polystyrene latex (PSL) nanoparticle (NP) sample is one of the most widely used standard materials. It is used for calibration of particle counters and particle size measurement tools. It has been reported that the measured NP sizes by various methods, such as Differential Mobility Analysis, dynamic light scattering (DLS), optical microscopy (OM), scanning electron microscopy (SEM) and atomic force microscopy (AFM), differ from each other. Deformation of PSL NPs on mica substrate has been reported in AFM measurements: the lateral width of PSL NPs is smaller than their vertical height. To provide a reliable calibration standard, the deformation must be measured by a method that can reliably visualize the entire three dimensional (3D) shape of the PSL NPs. Here we present a method for detailed measurement of PSL NP 3D shape by means of electron tomography in a transmission electron microscope. The observed shape of the PSL NPs with 100 nm and 50 nm diameter were not spherical, but squished in direction perpendicular to the support substrate by about 7.4% and 12.1%, respectively. The high difference in surface energy of the PSL NPs and that of substrate together with their low Young modulus appear to explain the squishing of the N...Continue Reading

References

Jul 1, 1992·Journal of Microscopy·D P BarnardB F McEwen
May 7, 2013·Micron : the International Research and Review Journal for Microscopy·M HayashidaS Ogawa

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Citations

Oct 22, 2016·Analytical and Bioanalytical Chemistry·Ravi Kiran Attota, Eileen Cherry Liu
Oct 25, 2016·Micron : the International Research and Review Journal for Microscopy·Misa Hayashida, Marek Malac
Aug 23, 2019·Beilstein Journal of Nanotechnology·Loïc CrouzierNicolas Feltin
Nov 21, 2020·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Misa HayashidaKiichi Fukui

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