Three-dimensional alginate hydrogels for radiobiological and metabolic studies of cancer cells

Colloids and Surfaces. B, Biointerfaces
Graham H ReadAparna H Kesarwala

Abstract

The purpose of this study is to demonstrate calcium alginate hydrogels as a system for in vitro radiobiological and metabolic studies of cancer cells. Previous studies have established calcium alginate as a versatile three-dimensional (3D) culturing system capable of generating areas of oxygen heterogeneity and modeling metabolic changes in vitro. Here, through dosimetry, clonogenic and viability assays, and pimonidazole staining, we demonstrate that alginate can model radiobiological responses that monolayer cultures do not simulate. Notably, alginate hydrogels with radii greater than 500 μm demonstrate hypoxic cores, while smaller hydrogels do not. The size of this hypoxic region correlates with hydrogel size and improved cell survival following radiation therapy. Hydrogels can also be utilized in hyperpolarized magnetic resonance spectroscopy and extracellular flux analysis. Alginate therefore offers a reproducible, consistent, and low-cost means for 3D culture of cancer cells for radiobiological studies that simulates important in vivo parameters such as regional hypoxia and enables long-term culturing and in vitro metabolic studies.

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Citations

Oct 17, 2019·Annals of Biomedical Engineering·Logan A NorthcuttMarjan Rafat
Apr 11, 2021·Advanced Drug Delivery Reviews·Sabina PozziRonit Satchi-Fainaro
Aug 28, 2021·Cancers·Melanie Kappelmann-FenzlAnja K Bosserhoff
Aug 22, 2021·Gastroenterology·Palanisamy NallasamyMoorthy P Ponnusamy
Dec 29, 2021·Molecular Carcinogenesis·Graham H ReadAparna H Kesarwala

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