Three-dimensional architecture of common benign and precancerous prostate epithelial lesions

Histopathology
Esther I VerhoefGeert J L H van Leenders

Abstract

Many glandular lesions can mimic prostate cancer microscopically, including atrophic glands, adenosis and prostatic intraepithelial neoplasia. While the characteristic histopathological and immunohistochemical features of these lesions have been well established, little is known about their three-dimensional architecture. Our objective was to evaluate the three-dimensional organisation of common prostate epithelial lesions. 500 μm-thick punches (n = 42) were taken from radical prostatectomy specimens, and stained with antibodies targeting keratin 8-18 and keratin 5 for identification of luminal and basal cells, respectively. Tissue samples were optically cleared in benzyl alcohol:benzyl benzoate and imaged using a confocal laser scanning microscope. The three-dimensional architecture of peripheral and transition zone glands was acinar, composed of interconnecting and blind-ending saccular tubules. In simple atrophy, partial atrophy and post-atrophic hyperplasia, the acinar structure was attenuated with branching blind-ending tubules from parental tubular structures. Three-dimensional imaging revealed a novel variant of prostate atrophy characterised by large Golgi-like atrophic spaces parallel to the prostate surface, which wer...Continue Reading

References

Dec 16, 1998·Virchows Archiv : an International Journal of Pathology·B Helpap
Sep 17, 1999·The American Journal of Pathology·J A DollH Donis-Keller
Sep 10, 2002·The American Journal of Surgical Pathology·Rajal B ShahMark A Rubin
Feb 21, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·John R Srigley
Dec 13, 2005·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Mildred B Martens, Jeremy H Keller
Sep 27, 2006·The American Journal of Surgical Pathology·Angelo M De MarzoM S Lucia
Sep 21, 2007·Histopathology·D M BerneyUNKNOWN Trans-Atlantic prostate group
Jan 4, 2012·Histopathology·Jonathan I Epstein
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
May 15, 2013·Human Pathology·Whitney M GreenJonathan I Epstein
Jul 28, 2013·The American Journal of Surgical Pathology·Liang ChengShaobo Zhang
Dec 1, 2008·Surgical Pathology Clinics·George J Netto, Jonathan I Epstein
Jun 30, 2016·Histopathology·Martin E van RoyenGeert J L H van Leenders
Jan 4, 2018·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Ming Zhou
Feb 10, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Esther I VerhoefGeert J L H van Leenders

❮ Previous
Next ❯

Citations

Oct 20, 2020·Pathology·Lars EgevadHemamali Samaratunga
Oct 31, 2021·Nature Protocols·Tariq Sami HaddadIris Nagtegaal

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
biopsy
imaging techniques
confocal microscopy

Software Mentioned

Fiji
Amira
ImageJ
Statistical Package for Social Sciences ( SPSS
Huygens Professional

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.