Three-dimensional architecture of hair-bundle linkages revealed by electron-microscopic tomography.

Journal of the Association for Research in Otolaryngology : JARO
Manfred AuerA J Hudspeth

Abstract

The senses of hearing and balance rest upon mechanoelectrical transduction by the hair bundles of hair cells in the inner ear. Located at the apical cellular surface, each hair bundle comprises several tens of stereocilia and a single kinocilium that are interconnected by extracellular proteinaceous links. Using electron-microscopic tomography of bullfrog saccular sensory epithelia, we examined the three-dimensional structures of basal links, kinociliary links, and tip links. We observed significant differences in the appearances and dimensions of these three structures and found two distinct populations of tip links suggestive of the involvement of different proteins, splice variants, or protein-protein interactions. We noted auxiliary links connecting the upper portions of tip links to the taller stereocilia. Tip links and auxiliary links show a tendency to adopt a globular conformation when disconnected from the membrane surface.

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Citations

Jan 22, 2013·Nature Neuroscience·Jung-Bum ShinPeter G Barr-Gillespie
Mar 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Florin VranceanuAnna Lysakowski
Jul 28, 2009·Current Opinion in Otolaryngology & Head and Neck Surgery·Hirofumi SakaguchiBechara Kachar
Jan 4, 2013·PloS One·Kateri J Spinelli, Peter G Gillespie
Aug 7, 2014·Nature Reviews. Neuroscience·A J Hudspeth
Feb 9, 2012·Physiology·Lifeng Pan, Mingjie Zhang
Apr 27, 2012·Physiological Reviews·Shinji Hirano, Masatoshi Takeichi
Apr 18, 2019·Journal of Neurophysiology·Jong-Hoon NamE H Peterson
May 11, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tobias F BartschA J Hudspeth
May 4, 2013·Journal of Cell Science·Carole M Hackney, David N Furness
Nov 9, 2021·Frontiers in Cell and Developmental Biology·Maryna V IvanchenkoDavid P Corey

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