Three dimensional association of double-stranded helices are produced in conditions for Z-DNA formation

Journal of Biomolecular Structure & Dynamics
B RevetA Niveleau

Abstract

The Z form of alternating poly(dG-dC).poly(dG-dC) can be induced when the concentration of NaCl, MgCl2 or ethanol are increased. In order to obtain more information concerning this Z structure, the B----Z transition is analyzed on the same sample, both by UV spectrophotometry and electron microscopy. The procedures used in this work provide high resolution images with minimal alterations of the molecules. It is shown that at high values of cations or ethanol, the polymer makes complex associations of numerous molecules stuck together parallelly. By decreasing the salt or ethanol concentrations, a progressive decondensation of the molecules is obtained. At low concentrations of Mg++ (2.10(-2) M), alterations of the linear secondary structure of the molecules are observed, although the UV spectrum is of the B-type. In the presence of that low concentration of Mg++, natural DNAs (phi X174 and yeast mitochondrial DNA fragment inserted in pBR) exhibit structural modifications similar to those observed with the poly(dG-dC).poly(dG-dC). These structures mainly consist in four-stranded hairpins and loops built up by the sticking of two segments of DNA. The correlation between these intertwining of short DNA segments and the presence of...Continue Reading

References

Mar 25, 1976·Nature·F M Pohl
Aug 5, 1973·Journal of Molecular Biology·D Lang
Apr 1, 1971·Journal of Ultrastructure Research·J DubochetE Kellenberger
Sep 14, 1981·FEBS Letters·B Malfoy, M Leng
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·F D MillerJ B Rattner
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·D B Haniford, D E Pulleyblank
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·H Castleman, B F Erlanger
Mar 25, 1983·Nucleic Acids Research·T J Thomas, V A Bloomfield
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J L CrawfordA Rich
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E M LaferA Rich
Jan 1, 1982·Annual Review of Biochemistry·S B Zimmerman
Nov 11, 1982·Nucleic Acids Research·M VorlíckováJ Sponar
Apr 30, 1981·Biochemical and Biophysical Research Communications·J KyprV Sklenár
Feb 21, 1980·Nature·S ArnottR L Ratliff
Aug 7, 1980·Nature·H DrewR E Dickerson

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Citations

Aug 26, 1986·Nucleic Acids Research·T J Thomas, R P Messner
Feb 11, 1987·Nucleic Acids Research·B Theveny, B Revet
Jan 1, 1990·Biopolymers·G E PlumV A Bloomfield
Jun 1, 1988·Journal of Biomolecular Structure & Dynamics·J B Chaires, M T Norcum
Feb 1, 1992·Journal of Biomolecular Structure & Dynamics·E Morier-TeissierE Delain
Dec 1, 1996·Quarterly Reviews of Biophysics·Y Timsit, D Moras

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