Three-dimensional cell culture: a breakthrough in vivo

International Journal of Molecular Sciences
Delphine AntoniGeorges Noel

Abstract

Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. As compared to the two-dimensional case, the three-dimensional (3D) cell culture allows biological cells to grow or interact with their surroundings in all three dimensions thanks to an artificial environment. Cells grown in a 3D model have proven to be more physiologically relevant and showed improvements in several studies of biological mechanisms like: cell number monitoring, viability, morphology, proliferation, differentiation, response to stimuli, migration and invasion of tumor cells into surrounding tissues, angiogenesis stimulation and immune system evasion, drug metabolism, gene expression and protein synthesis, general cell function and in vivo relevance. 3D culture models succeed thanks to technological advances, including materials science, cell biology and bioreactor design.

References

Oct 27, 1997·Annals of Plastic Surgery·M M GhoshS Mac Neil
Aug 13, 1998·Nature Medicine·B R Unsworth, P I Lelkes
Jun 28, 2000·Journal of Cancer Research and Clinical Oncology·F Castañeda, R K Kinne
Jun 14, 2002·Biochemical and Biophysical Research Communications·Shuji TodaHajime Sugihara
Aug 22, 2003·Nature·Alison Abbott
Feb 6, 2004·Trends in Biotechnology·Ivan MartinMichael Heberer
Apr 2, 2004·Nature·Robert Langer, David A Tirrell
Dec 20, 2005·Gene·Janet RubinChristopher Rae Jacobs
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Linda G Griffith, Melody A Swartz
Feb 23, 2007·Nature·Sheila MacNeil
Aug 9, 2007·Nature Reviews. Molecular Cell Biology·Francesco PampaloniErnst H K Stelzer
Oct 12, 2007·Biotechnology and Bioengineering·Tao SunJohn W Haycock
May 6, 2008·Tissue Engineering. Part B, Reviews·Jungwoo LeeNicholas A Kotov
Jun 21, 2008·Biotechnology Journal·Ruei-Zeng LinHwan-You Chang
Jun 2, 2009·Biochemical Pharmacology·Bharat B AggarwalPrashasnika Gehlot
Apr 7, 2010·Nature Reviews. Drug Discovery·William N Hait
Sep 8, 2010·Seminars in Oncology·Vernon E Steele, Ronald A Lubet
Mar 31, 2011·Nature Reviews. Clinical Oncology·Lisa Hutchinson, Rebecca Kirk
Jun 24, 2011·Cytotherapy·Fatima A Saleh, Paul G Genever
Dec 14, 2011·Biotechnology and Bioengineering·Amy Y HsiaoShuichi Takayama
Aug 16, 2012·Cancer Biology & Therapy·Federico Perche, Vladimir P Torchilin
Sep 6, 2012·Biotechnology and Bioengineering·Abigail D BellisLonnie D Shea

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Citations

Feb 13, 2016·Assay and Drug Development Technologies·Sarah-Louise RyanAnthony M Davies
Jun 30, 2015·Assay and Drug Development Technologies·Richard M Eglen, David H Randle
May 10, 2016·BioResearch Open Access·Sara ShafaieDavid Y S Chau
Oct 21, 2016·Expert Opinion on Drug Safety·Yimu ZhaoMilica Radisic
Sep 16, 2016·PloS One·Yi Wen ChaiJohn H Brekke
Nov 5, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Khatereh Saei ArezoumandNosratollah Zarghami
Dec 23, 2016·Tissue Engineering. Part C, Methods·Mohammad HoushmandMahin Nikougoftar Zarif
May 17, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Seyede Elmira Yazdi RouholaminiKobra Omidfar
Apr 14, 2017·Molecular Reproduction and Development·Jeremy R MilesAngela K Pannier
Jan 14, 2017·Photochemistry and Photobiology·Luca CeveniniAldo Roda
Jun 29, 2017·The International Journal of Biochemistry & Cell Biology·Janine AucampPiet J Pretorius
May 28, 2017·The Journal of Biological Chemistry·Sona Lakshme BalasubramaniamSonali P Barwe
Jun 16, 2017·Journal of Cellular Physiology·Eddy-Tim VerjansLiliane Schoofs
Aug 16, 2016·Journal of Tissue Engineering·Kayla F GoliwasAndra R Frost
Oct 1, 2016·Reproduction in Domestic Animals = Zuchthygiene·M G MorselliG C Luvoni
Apr 6, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jessica E KimMichael Mak
Feb 25, 2017·Acta Obstetricia Et Gynecologica Scandinavica·Erin GreavesPhilippa T K Saunders
Dec 12, 2017·Proteomics. Clinical Applications·Mitchell AclandPeter Hoffmann
Apr 27, 2018·Lab on a Chip·Ian C McLeanCharles S Henry
Nov 28, 2017·Scientific Reports·Rose N NjorogeSarki A Abdulkadir
May 31, 2018·Tissue Engineering. Part a·Josephine LembongJohn P Fisher
Jun 8, 2018·Advanced Materials·Teresa Simon-YarzaChristian Serre
Jun 8, 2018·PloS One·Corina FrickMatthias Mehling
Sep 20, 2018·Journal of Applied Toxicology : JAT·Elise K TiganiGili Kaufman
Sep 23, 2018·Journal of Biomedical Materials Research. Part a·Xiao-Fang TongQiang-Song Wang
Nov 30, 2018·Expert Opinion on Drug Metabolism & Toxicology·Gerhard Hamilton, Barbara Rath
Nov 6, 2018·Macromolecular Rapid Communications·Gijs RamakersTanja Junkers
Nov 13, 2018·NPJ Systems Biology and Applications·Greta Del MistroDagmar Kulms
Nov 30, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xiaoyang LiZhenfeng Duan

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Methods Mentioned

BETA
confocal microscopy
xenografts

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