Three Dimensional Conjugation of Recombinant N-Cadherin to a Hydrogel for In Vitro Anisotropic Neural Growth

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Johana C M Vega LHyunjoon Kong

Abstract

Living cells are extensively being studied to build functional tissues that are useful for both fundamental and applied bioscience studies. Increasing evidence suggests that cell-cell adhesion controlled by intercellular cadherin junction plays important roles in the quality of the resulting engineered tissue. These findings prompted efforts to interrogate biological effects of cadherin at a molecular scale; however, few efforts were made to harness the effects of cadherin on cells cultured in an in vivo-like three dimensional matrix. To this end, this study reports a hydrogel matrix three dimensionally functionalized with a controlled number of Fc-tagged recombinant N-cadherins (N-Cad-Fc). To retain the desired conformation of N-Cad, these cadherins were immobilized and oriented to the gel by anti-Fc-antibodies chemically coupled to gels. The gels were processed to present N-Cad-Fc in uniaxially aligned microchannels or randomly oriented micropores. Culturing cortical cells in the functionalized gels generated a large fraction of neurons that are functional as indicated by increased intracellular calcium ion concentrations with the microchanneled gel. In contrast, direct N-Cad-Fc immobilization to microchannel or micropore wal...Continue Reading

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Citations

Jan 24, 2018·Advanced Healthcare Materials·Mi Y KwonJason A Burdick
Oct 17, 2017·Angewandte Chemie·Koki SanoTakuzo Aida
Mar 20, 2019·Advanced Science·Xiaoxia LeTao Chen
May 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yinghong ZhouYin Xiao
Feb 16, 2021·Advanced Healthcare Materials·Eva Barcelona-EstajeManuel Salmeron-Sanchez
Mar 19, 2021·Neurochemistry International·Dimitri EigelBen Newland
Apr 20, 2021·APL Bioengineering·Mai T Ngo, Brendan A C Harley
Jul 16, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Harsimran Kaur, Sangita Roy
Aug 7, 2021·Acta Biomaterialia·Fiona R PassanhaVanessa L S LaPointe

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