Three dimensional culture of HepG2 liver cells on a rat decellularized liver matrix for pharmacological studies

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Kamal H HusseinHeung M Woo

Abstract

Three-dimensional in vitro tumor models are needed to obtain more information about drug behavior in tumors. The aim of this study is to establish a new model for hepatocellular carcinoma (HCC) using decellularized rat livers. After generating the rat liver scaffolds, HepG2 liver cancer cells were perfused via the portal vein and placed in a bioreactor for 10 days. Histology was performed to analyze cell distribution within the scaffolds. Function and tumor-related gene expression were examined by polymerase chain reaction (PCR). We evaluated the function of HepG2 cells grown on scaffolds in the presence of a well-known anti-cancer drug to investigate the potential application of our system for drug screening. The scaffolds were devoid of cellular materials and preserved extracellular matrix components. HepG2 cells grew well on the scaffolds. The PCR results showed that the cells maintained function and invasion ability at significantly higher levels than cells grown on two-dimensional (2-D) dishes or spheroids on Matrigel. Unlike the 2-D cultures, albumin secretion and alpha-fetoprotein expression in three-dimensional cultures were less susceptible to lower concentrations of the drug. Cells grown in scaffolds seemed to respond...Continue Reading

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Citations

Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Kamal Hany HusseinHeung-Myong Woo
Mar 13, 2020·Journal of Biomaterials Science. Polymer Edition·Chaochen ZhaoYi Gao
Apr 25, 2019·Materials·Takashi Hoshiba
Sep 23, 2018·International Journal of Molecular Sciences·Dimakatso Alice SenthebaneKevin Dzobo
Aug 30, 2018·Stem Cells International·Kevin DzoboKeolebogile Shirley Caroline M Motaung
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Hermann B FrieboesBiana Godin
Oct 30, 2019·Bioengineering·Christina KryouIoanna Zergioti
Nov 1, 2020·Cancers·Alexander NeyStephen P Pereira
Dec 11, 2020·Scientific Reports·Manal Matoug-ElwerfelliMonty Duggal
May 12, 2021·Acta Biochimica Et Biophysica Sinica·Ting YaoXiaohua Chen
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Lei ChenXiansi Zeng
Jun 23, 2021·Acta Biomaterialia·Seokyoung BangHong Nam Kim
Aug 4, 2021·Cell Regeneration·Do Thuy Uyen Ha LamHuck-Hui Ng
Nov 5, 2021·Advanced Healthcare Materials·Maria RafaevaAlejandro E Mayorca-Guiliani

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