Three-dimensional culture of mouse bone marrow cells within a porous polymer scaffold: effects of oxygen concentration and stromal layer on expansion of haematopoietic progenitor cells

Journal of Tissue Engineering and Regenerative Medicine
Hirotoshi MiyoshiThein Tun

Abstract

To establish an ex vivo expansion method of haematopoietic progenitor cells (HPCs) and erythroid cells, three-dimensional (3D) cultures of mouse bone marrow cells were performed, employing a porous polyvinyl formal (PVF) resin as a scaffold. In these cultures, the effects of oxygen concentration and co-cultures with stromal cells on the expansion of HPCs and erythroid cells were investigated. When bone marrow cells were cultured under 3D conditions, HPCs and erythroid cells expanded without supplementation of exogenous cytokines, irrespective of the presence of stromal cells. On the contrary, slight expansion of HPCs or erythroid cells was observed in monolayer cultures as controls, indicating that the 3D cultures using the PVF scaffold were far better in expanding HPCs and erythroid cells than the monolayer cultures. Under hypoxic conditions, bone marrow stromal cells allowed for a 3D culture of erythroid cells and HPCs at higher cell densities compared to cultures without stromal cells, and the duration of the expansion of HPCs and erythroid cells after initiating the 3D co-cultures was prolonged. The number of these cells increased throughout the culture period up to 3 weeks under hypoxic conditions, although the number decr...Continue Reading

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Citations

Apr 4, 2013·Foot and Ankle Surgery : Official Journal of the European Society of Foot and Ankle Surgeons·Martinus Richter, Stefan Zech
Sep 12, 2015·Biotechnology Journal·Ji Sun ChoiBrendan A C Harley
Apr 21, 2017·Current Opinion in Hematology·Shivem B Shah, Ankur Singh
Mar 13, 2012·Current Opinion in Hematology·David J AnsteeAshley M Toye
Apr 17, 2012·Current Opinion in Hematology·Yi Shen, Susan K Nilsson
Jan 7, 2020·Nature Reviews. Materials·Sungwoong KimAnkur Singh
Feb 23, 2021·The International Journal of Artificial Organs·Hirotoshi MiyoshiYoshio Utsumi

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